D-pinitol mitigates tumor growth by modulating interleukins and hormones and induces apoptosis in rat breast carcinogenesis through inhibition of NF-κB

• Thamaraiselvan Rengarajan ^[1] ; Natarajan Nandakumar ^[2] ; Peramaiyan Rajendran ^[3] ; Mohanraj Karthik Ganesh ^[2] ; Maruthaiveeran Periyasamy Balasubramanian ^[2] ; Ikuo Nishigaki ^[3]
  1. [1] Universiti Sains Malaysia

     Universiti Sains Malaysia

     Malasia

     
  2. [2] University of Madras

     University of Madras

     India

     
  3. [3] NPO-International Laboratory of Biochemistry, Japon

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• Localización: Journal of physiology and biochemistry, ISSN-e 1877-8755, ISSN 1138-7548, Vol. 71, Nº. 2 (June), 2015, págs. 191-204
• Idioma: inglés
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  • Breast cancer is the most prevalent malignant neoplasm in the world, and chemoprevention through dietary intervention strategy is an emerging option to reduce the incidence. d-pinitol (DP), a major component of soya bean, possesses attractive biological actions. We have investigated whether d-pinitol have an effect on tumor growth in vivo against 7,12-dimethylbenz(a)anthracene (DMBA)-initiated rat mammary carcinogenesis and investigated its mechanism of action. Tumors were induced in Sprague–Dawley (SD) rats by a gastric dose of 20 mg/kg DMBA, and after 13 weeks of induction period, the rats were orally administered with d-pinitol for 45 days. At the end of the assay, animals in carcinogen control group prompted a tumor incidence of 100 % and developed a tumor volume of 8.35 ± 0.56, which was significantly reduced to 5.74 ± 0.32 for the animals treated with d-pinitol. The d-pinitol treatment not only decreased the tumor volume but also further examination revealed that tumors from animals that received d-pinitol reduced nuclear factor kappa B (NF-κB) activation which in turn results in modulation of its downstreaming p53 and proteins of caspase-3 family. Bcl-2 expression and caspase-3 activation were also decreased after d-pinitol supplementation leading to induction of apoptosis and finally cell death. Furthermore, the status of the inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin (IL)-2, IL-6, and tumor markers, lipid profile, and hormones was also significantly declined up on d-pinitol administration. Thus, it reveals the collective involvement of the abovementioned parameters along with NF-κB signaling through which d-pinitol induces apoptosis and subsequently suppresses breast cancer during DMBA-induced rat breast carcinogenesis.