Extraction and Purification of Glucoraphanin by Preparative High-Performance Liquid Chromatography (HPLC)

• Iris Lee ^[1] ; Mary C. Boyce ^[1]
  1. [1] Edith Cowan University

     Edith Cowan University

     Australia

     
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 88, Nº 6, 2011, págs. 832-834
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • A student activity that focuses on the isolation of glucoraphanin from broccoli using preparative high-performance liquid chromatography (HPLC) is presented here. Glucoraphanin is a glucosinolate, whose byproducts are known to possess anticancer properties. It is present naturally at high levels in broccoli and other Brassica vegetables. This activity has students practicing and developing a number of skills including liquid extraction; cleanup by solid-phase extraction; analytical HPLC with diode array detection to identify the peak of interest; and preparative HPLC coupled with a fraction collector to isolate the glucoraphanin fraction. It also represents an authentic application of preparative HPLC as glucoraphanin is not readily available from the main chemical suppliers.