Determination of Niacinamide in Lotions and Creams Using Liquid–Liquid Extraction and High-Performance Liquid Chromatography

• Karyn M. Usher ^[1] ; Carolyn R. Simmons ^[2] ; Daniel W. Keating ^[3] ; Henry F. Rossi ^[3]
  1. [1] Metropolitan State University

     Metropolitan State University

     City of Saint Paul, Estados Unidos

     
  2. [2] Spring Hill College

     Spring Hill College

     Estados Unidos

     
  3. [3] Department of Chemistry, West Chester University of Pennsylvania, Estados Unidos

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• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 92, Nº 5, 2015, págs. 907-910
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Chemical separations are an important part of an undergraduate chemistry curriculum. Sophomore students often get experience with liquid–liquid extraction in organic chemistry classes, but liquid–liquid extraction is not as often introduced as a quantitative sample preparation method in honors general chemistry or quantitative analysis classes. This experiment offers a relevant, interesting way to use liquid–liquid extraction and high-performance liquid chromatography (HPLC) to analyze popular lotions and creams for niacinamide content. After completing the experiment, students will have a better understanding of the extraction technique, Beer–Lambert law, and HPLC. The percent recovery from a spiked sample of Olay Original Active Hydrating Beauty Fluid was found to be 99.22% with a standard deviation of 0.01%.