Identification and Quantitative Analysis of Acetaminophen, Acetylsalicylic Acid, and Caffeine in Commercial Analgesic Tablets by LC−MS

• Christopher J. Fenk ^[1] ; Nicole M. Hickman ^[3] ; Melissa A. Fincke ^[3] ; Douglas H. Motry ^[1] ; Barry Lavine ^[2]
  1. [1] Kent State University

     Kent State University

     City of Kent, Estados Unidos

     
  2. [2] Oklahoma State University

     Oklahoma State University

     Estados Unidos

     
  3. [3] University Tuscarawas Campus, Ohio

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• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 87, Nº 8 (August), 2010, págs. 838-841
• Idioma: inglés
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• Resumen

  • An undergraduate LC−MS experiment is described for the identification and quantitative determination of acetaminophen, acetylsalicylic acid, and caffeine in commercial analgesic tablets. This inquiry-based experimental procedure requires minimal sample preparation and provides good analytical results. Students are provided sufficient background information to allow them to independently develop chromatographic separation and analytical-analysis procedures using a liquid chromatograph−mass spectrometer. The experiment can be conducted in two consecutive, 3-h laboratory periods. Students gain experience using state-of-the-art analytical instrumentation that simultaneously provides chromatographic, ultraviolet, and mass spectrometry data. The efficiency of concurrently collecting three different types of experimental data translates into more time for inquiry-based investigation, instructional emphasis on theory, and data manipulation.