Resumen de FR 01. In vitro fertilization of oocytes colected postmortem from zebu cows
Adriana Hernández, Pedro Bastidas, Juan Troconiz
Studies were conducted to establish and validate an in vitro fertilization system (IVF) at the Institute of Animal Reproduction and A.I., Faculty of Veterinary Sciences, Central University of Venezuela. Oocytes (n=748) were aspirated from different size follicles collected from ovaries of Zebu cows, from the 748 oocytes, 344 had cumulus cells while the remaining (404) were denuded oocytes. The maturation media utilized was TCM-199. A study included the oocyte TCM199 media culture supplemented with 0.8 µg/mL of GnRH (n=360) and a control group (n=388) without GnRH. Frozen semen from bulls of renown fertility was used and capacited with heparin (10µl/mL). There were statistical differences (P.05) due to the presence or absence of cumulus cells. Overall these results indicate that the IVF system employed was effective allowing an overall mean of 70 % for fertilization rate across replicates. Result of these studies also indicate that the IVF technique developed and validated utilizing an homologous system allowed for incorporating a laboratory methodology for predicting semen potential fertility of bulls from different breeds.
