Resumen de Formación de biopelículas en el hongo patógeno "Sporothrix schenckii": desarrollo, arquitectura y características bioquímicas

Rocío Sánchez Herrera, Lérida Liss Flores Villavicencio, Israel Padilla Guerrero, Gloria Barbosa Sabanero, Myrna Sabanero López

• español

  Las infecciones fungicas en el ser humano estan relacionadas con la formacion de biopeliculas resistentes a tratamientos farmacologicos. El complejo de S. schenckii presenta micosis cronicas y sistemicas con resistencia farmacologica. Sin embargo, en S. schenckii no se ha descrito la formacion de biopeliculas; este aspecto se aborda en la presente investigacion, analizando las caracteristicas bioquimicas de la formacion y desarrollo de las biopeliculas, aplicando tecnicas de microscopia optica y metodos fisicoquimicos. Los resultados indican que la adhesion y desarrollo de la comunidad fungica es tiempo dependiente (12 h, 24 h y 48 h); consecuentemente se presenta incremento de la biomasa (0.19 Abs - 0.5 Abs) y carbohidratos totales (1.12 �Êg/�Êl - 3.28 �Êg/�Êl). Estructuralmente, la adhesion y desarrollo de S.

  schenckii presenta redes de hifas con centros de nucleacion que consolidan la biopelicula.

  La prospectiva del estudio de las biopeliculas de S. schenckii impactara en las estrategias farmacologicas del tratamiento de las micosis

• English

  Fungal infections of the human being are associated to the formation of biofilms, which are resistant to antifungal drug therapy. The complex of S. schenckii presents chronic and systemic mycoses with antifungal resistance. However, in S. schenckii the biofilm formation has not been described, this issue is addressed in the present research, analyzing the biochemical characteristics of the formation and development of biofilms, using optical microscopy and physicochemical methods. The results indicate that the adhesion and development of the fungal community is time dependent (12 h, 24 h and 48 h). The results indicate that the adhesion and development of the fungal community is time dependent (12 h, 24 h and 48 h), thereby increasing the biomass (0.19 Abs to 0.5 Abs) and total carbohydrates (1.12 ìg/ìl - 3.28 ìg/ìl) occurs. When adhesion and development takes place, S. schenckii has hyphae networks with nucleation centers that consolidate the biofilm structurally. Prospective study of biofilms of S. schenckii impact in the pharmacological treatment strategies of fungal infectionsFungal infections of the human being are associated to the formation of biofilms, which are resistant to antifungal drug therapy. The complex of S. schenckii presents chronic and systemic mycoses with antifungal resistance. However, in S. schenckii the biofilm formation has not been described, this issue is addressed in the present research, analyzing the biochemical characteristics of the formation and development of biofilms, using optical microscopy and physicochemical methods. The results indicate that the adhesion and development of the fungal community is time dependent (12 h, 24 h and 48 h). The results indicate that the adhesion and development of the fungal community is time dependent (12 h, 24 h and 48 h), thereby increasing the biomass (0.19 Abs to 0.5 Abs) and total carbohydrates (1.12 ìg/ìl - 3.28 ìg/ìl) occurs. When adhesion and development takes place, S. schenckii has hyphae networks with nucleation centers that consolidate the biofilm structurally. Prospective study of biofilms of S. schenckii impact in the pharmacological treatment strategies of fungal infections