Scanning Electron Microscopy and Fourier Transformed Infrared Spectroscopy Analysis of Bone Removal Using Er:: YAG and CO2 Lasers
- Autores: Katia M. Sasaki, Akira Aoki Paula Eduardo, Shizuko Ichinose, Toshiaki Yoshino, Sachiko Yamada, Isao Ishikawa
- Localización: Journal of periodontology, ISSN 0022-3492, Vol. 73, Nº. 6, 2002, págs. 643-652
- Idioma: inglés
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Resumen
Background: A thorough analysis of laser-ablated bone tissue is required before applying the technique to osseous surgery. In this study, we examine the morphological features and chemical composition of the bone surface after Er:YAG and CO2 lasers ablation.
Methods: Six Wistar rats were used. An Er:YAG laser was used for ablation at an output energy of 100 mJ/pulse and a pulse rate of 10 Hz (1 W). Continuous CO2 laser irradiation was performed at an output energy of 1 W. Sites drilled using a conventional micromotor were used as controls. Analysis using scanning electron microscopy (SEM) and Fourier transformed infrared (FTIR) spectroscopy was performed.
Results: Er:YAG laser ablation produced a groove with similar dimensions to that produced by bur drilling, whereas the CO2 laser produced only a charred line with minimal tissue removal. SEM observations revealed that the groove produced by the Er:YAG laser had well-defined edges and a smear layerfree surface with a characteristically rough appearance and with entrapped fibrin-like tissue. The melting and carbonization produced by the CO2 laser were not observed on sites irradiated by the Er:YAG laser. FTIR spectroscopy revealed that the chemical composition of the bone surface after Er:YAG laser ablation was much the same as that following bur drilling. The production of toxic substances that occurred after CO2 laser irradiation was not observed following Er:YAG laser irradiation or bur drilling.
Conclusion: These results suggest that the use of Er:YAG laser ablation may become an alternative method for oral and periodontal osseous surgery. J Periodontol 2002;73:643-652.
