Subgingival Microbiota of Brazilian Subjects With Untreated Chronic Periodontitis
- Autores: Ana Paula Vieira Colombo, Ricardo P. Teles, Maria Cynésia Torres, Renata Souto, Wilson Rosalém, Maria Cláudia S. Mendes, Milton Uzeda
- Localización: Journal of periodontology, ISSN 0022-3492, Vol. 73, Nº. 4, 2002, págs. 360-369
- Idioma: inglés
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Resumen
Background: Different periodontopathogenic microbiota have been associated with periodontal diseases in several populations. The present investigation determined the subgingival microbiota of untreated chronic periodontitis Brazilians using the checkerboard DNA-DNA hybridization technique.
Methods: Twenty-five periodontitis patients (mean age, 41 ± 2; mean probing depth [PD], 3.3 ± 0.2; mean attachment level [AL], 3.6 ± 0.2) with no history of previous periodontal therapy and a control group of 14 healthy subjects (mean age, 34 ± 0.6; mean PD, 1.8 ± 0.2; mean AL, 1.7 ± 0.1) were selected. Measurements of PD, AL, bleeding on probing, plaque accumulation, and suppuration were recorded at 6 sites/tooth. Subgingival plaque samples were obtained from 4 sites in each tooth/subject in both groups. The presence and levels of 41 subgingival species were determined in 4,032 plaque samples using whole genomic DNA probes and the checkerboard method.
Results: Periodontal pathogens, as well as some unusual species (E. faecalis, E. coli and Bartonella sp.), were detected significantly more often and/or in higher levels in the periodontitis group (P <0.05). Most species were more frequently detected in interproximal sites. B. forsythus, P. gingivalis, E. nodatum, and F. nucleatum ss vincentii showed a significant positive correlation with mean PD and AL (P <0.05).
Conclusions: The subgingival microbiota of Brazilians with untreated chronic periodontitis were complex, including high proportions of periodontopathogens commonly found in other populations, as well as some unusual species. J Periodontol 2002;73:360-369.
