Effect of Recombinant Human Bone Morphogenetic Protein-4 Dose on Bone Formation in a Rat Calvarial Defect Model

• Autores: Eun-Kyoung Pang, Se-Ung Im, Chang-Sung Kim, Seong-Ho Choi, Jung-Kiu Chai, Chong-Kwan Kim, Soo-Boo Han, Kyoo-Sung Cho
• Localización: Journal of periodontology, ISSN 0022-3492, Vol. 75, Nº. 10, 2004, págs. 1364-1370
• Idioma: inglés
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  • Background: Bone morphogenetic proteins (BMPs) are being evaluated for periodontal and bone regenerative therapy. The objective of this study was to evaluate the effect of recombinant human bone morphogenetic protein-4 (rhBMP-4) dose on local bone formation in a rat calvaria defect model.

    Methods: Calvarial, 8 mm diameter, critical-size osteotomy defects were created in 140 male Sprague-Dawley rats. Seven groups of 20 animals each received either 1) rhBMP-4 (2.5 µg) in an absorbable collagen sponge (ACS) carrier, 2) rhBMP-4 (5 µg)/ACS, 3) rhBMP-4 (2.5 µg) in a β-tricalcium phosphate (β-TCP) carrier, 4) rhBMP-4 (5 µg)/β-TCP, 5) ACS or 6) β-TCP carrier controls, or 7) a sham-surgery control, and were evaluated by histologic and histometric parameters following a 2- or 8-week healing interval (10 animals/group/healing interval).

    Results: Surgical implantation of rhBMP-4/ACS and rhBMP- 4/β-TCP resulted in enhanced local bone formation at both 2 and 8 weeks. Within the dose range examined, rhBMP-4 did not exhibit an appreciable dose-dependent response. Defect closure was not significantly different between the rhBMP-4/ACS and rhBMP-4/β-TCP groups. New bone area of the rhBMP-4/ β-TCP group was significantly greater than that of the rhBMP-4/ ACS group; however, bone density in the rhBMP-4/ACS group was significantly greater than that in the rhBMP-4/β-TCP group at 8 weeks (P <0.05).

    Conclusions: rhBMP-4 combined with ACS or β-TCP has a significant potential to induce bone formation in the rat calvaria defect model. Within the selected rhBMP-4 dose range and observation interval, there appeared to be no meaningful differences in bone formation. J Periodontol 2004;75:1364-1370.