Specific Pro-Apoptotic Fibronectin Fragments Modulate Proteinase Expression in Periodontal Ligament Cells

• Autores: Shaohui Wang, Yvonne Kapila, Sang-Wook Jee
• Localización: Journal of periodontology, ISSN 0022-3492, Vol. 75, Nº. 4, 2004, págs. 523-530
• Idioma: inglés
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• Resumen

  • Specific Pro-Apoptotic Fibronectin Fragments Modulate Proteinase Expression in Periodontal Ligament Cells Sang-Wook Jee Department of Stomatology, School of Dentistry, University of California, San Francisco, CA.

    Shaohui Wang Department of Stomatology, School of Dentistry, University of California, San Francisco, CA.

    Dr. Yvonne L. Kapila Department of Stomatology, School of Dentistry, University of California, San Francisco, CA.

    Methods: We used substrate zymography, reverse zymography, proteinase inhibitors, and partial sequencing to investigate whether the pro-apoptotic V+H– fibronectin fragment modulates proteinase expression as part of this apoptotic mechanism.

    Results: Incubation with the V+H– fragment reduced the expression of all gelatinolytic proteinases and inhibitors commonly expressed by periodontal ligament cells. In the presence of caspase inhibitors, inhibitors known to suppress apoptosis, however, the reduced proteinase profile was rescued, showing that caspase inhibitors were able to reverse the reduced proteinase profile and indicating that caspase-mediated pathways are pertinent to fibronectin-fragment-mediated matrix metalloproteinase expression. In addition, the V+H– fragment also triggered the expression of a unique high molecular weight gelatinolytic proteinase of approximately 200 kDa. This proteinase was a serine proteinase, whose identity is not known.

    Conclusion: These findings suggest that matrix-degrading proteinases may be involved in apoptosis as part of a unique mechanism of periodontal tissue breakdown, in which novel proteinases may help execute the dissolution of the extracellular matrix. J Periodontol 2004;75:523-530.

    KEYWORDS: Apoptosis, metalloproteinases, matrix, periodontal ligament, serine proteinases, zymography