Resumen de Enhanced Cementum Formation in Experimentally Induced Cementum Defects of the Root Surface with the Application of Recombinant Basic Fibroblast Growth Factor in Collagen Gel In Vivo

Masato Tamura, Yoshinori Kuboki, Dr. Yasuko Sato, Masahiro Kikuchi, Noboru Ohata

• Enhanced Cementum Formation in Experimentally Induced Cementum Defects of the Root Surface with the Application of Recombinant Basic Fibroblast Growth Factor in Collagen Gel In Vivo Dr. Yasuko Sato Department of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.

  Masahiro Kikuchi Department of Operative Dentistry and Endodontics, School of Dentistry, Iwate Medical University, Morioka, Japan Noboru Ohata Department of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.

  Masato Tamura Department of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University.

  Yoshinori Kuboki Koken Bioscience Institute, Tokyo, Japan Background: Therapies using biologically active, soluble factors such as growth factors or cytokines have been investigated for potential clinical use in regenerating lost periodontal tissue due to periodontitis. Basic fibroblast growth factor (bFGF, FGF-2) is a multifunctional growth factor that has a variety of effects including induction of proliferation and morphogenesis in a wide range of cells and tissues including periodontal ligament tissue.

  Methods: In this study, we examined the effects of bFGF on the regeneration of cementum and periodontal ligament in experimentally induced partial defects in a beagle dog model. bFGF in a collagen gel was applied to the defects and root surfaces, and the teeth were replanted.

  Results: Eight weeks post-surgery, formation of cementum on denuded dentin was enhanced by application of 0.1, 1, or 5 μg of bFGF in a collagen gel compared to collagen gel containing vehicle. Histological analyses revealed that at 4 weeks post-surgery, random periodontal ligament fibers had bound to dentin, but were attached only to denuded dentin to which 0.1, 1, or 5 μg of bFGF in collagen gel had been applied. At 8 weeks post-surgery, we observed the formation of dense fibers bound to alveolar bone and newly synthesized cementum in teeth treated with 1 μg of bFGF.

  Conclusion: These results suggest that basic fibroblast growth factor in a collagen gel is a suitable therapy for damaged periodontal ligament and could lead to readily achievable methods of treatment for periodontal disease. J Periodontol 2004;75:243-248.

  KEYWORDS: Animal studies, collagen, dental cementum, growth factors, fibroblast, periodontal diseases/therapy, periodontal ligament