Eradication of enterococci biofilms by lactic acid alone and combined with chlorhexidine and cetrimide

• Autores: María Teresa Arias Moliz, Pilar Baca García, Santiago Ordóñez Becerra, Mª Paloma González Rodríguez, Carmen María Ferrer Luque
• Localización: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, ISSN-e 1698-6946, Vol. 17, Nº. 5 (septiembre), 2012
• Idioma: inglés
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• Resumen

  • Objective: The antimicrobial activity of lactic acid (LA) alone or in combination with chlorhexidine (CHX) and cetrimide (CTR) against three Enterococcus faecalis strains, E. faecalis ATCC 29212, E. faecalis EF- D1 and E.

    faecalis U-1765, one Enterococcus durans strain and one dual- species biofilm was investigated.

    Study Design: The irrigating solutions tested were 20%, 15%, 10%, 5% and 2.5% LA, alone and in combination with 2% CHX and with 0.2% CTR. The biofilms were grown in the MBEC TM high-throughput device for 24 hours and exposed to the solutions for 30 seconds and 1 minute. “Eradication” was defined as 100% bacterial kill.

    Results: Twenty percent LA eradicated all enterococci biofilms after 30 seconds contact time. The association of LA + 0.2% CTR achieved better results than LA alone, in contrast with the results obtained using LA + 2% CHX.

    E. durans was eradicated by all the tested solutions at 1 minute. The dual- species biofilm, E. faecalis ATCC 29212 + E. durans, gave intermediate values of the pure cultures.

    Conclusions: LA is capable of eradicating enterococci biofilm at a concentration of 20%. The combination of lower concentrations with 0.2% CTR achieved eradication after 1 minute.