Resumen de Lupinus albus, A Novel Vegetable Extract With Metalloproteinase Inhibitory Properties: A Potential Periodontal Therapy
Frédéric Galtier, Anne-Laure Ejeil, Sophie-Myriam Dridi, Philippe Msika, Bernard Pellat, Gaston Godeau
Background: In this study we examine the properties of a vegetable extract from seeds of Lupinus albus (LU105). In previous works we demonstrated that LU105 reduced the expression, by gingival fibroblasts, of both matrix metalloproteinase (MMP)-2 and MMP-9. We decided to study the impact of LU105 on cell proliferation and morphology. Using organ culture media we also studied the MMP and tissue inhibitors of metalloproteinases (TIMP) expression and the cytokines secretion.
Methods: Healthy and inflamed gingival biopsies were placed in appendage culture with or without LU105. The organ culture media were analyzed using Western blottings (MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, TIMP-1, and TIMP-2) and gelatine zymography. A reverse transcription polymerase chain reaction (RT-PCR) was also performed on healthy and inflamed gingival biopsies, which were maintained in culture with or without LU105 0.1%. Then, we decided to determine the amount of cytokines present in the organ culture media such as interleukin (IL)-1β, IL-4, IL-6, transforming growth factor (TGF)-β, and tumor necrosis factor (TNF)-α.
Results: When gingival biopsies derived from inflamed tissues were cultured with LU105 0.1% in the culture media, the MMP and TIMP expression and activity decreased significantly when compared to cultures without LU105. Moreover, we did not note any statistical difference in the cell proliferation compared with human gingival fibroblast cultures without LU105. Furthermore, IL-1β, IL-6, TGF-β, and TNF-α amounts in the culture media decreased significantly, whereas IL-4 increased significantly when LU105 0.1% was added to the culture media.
Conclusion: LU105, a novel metalloproteinase inhibitor with few consequences on cell proliferation and morphology, is a vegetable extract with potential clinical capacity. J Periodontol 2005;76:1329-1338.
