Effects of Endogenous and Exogenous Prostaglandin E2 on the Proliferation and Differentiation of a Mouse Cementoblast Cell Line (OCCM-30)

• Autores: Y. Maeda, M. Miyauchi, Martha J. Somerman, T. Takata
• Localización: Journal of periodontology, ISSN 0022-3492, Vol. 77, Nº. 12, 2006, págs. 2051-2058
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • Background: Cementum formation is considered to be a critical event for successful regeneration of periodontal tissues. Cementoblasts share many characteristics with osteoblasts. Prostaglandin E2 (PGE2) is an important local factor in bone metabolism. Although the effects of PGE2 on osteoblasts are well known, its effects on cementoblasts have not yet been established. We examined the effects of PGE2 on proliferation and differentiation in a mouse cementoblast cell line, OCCM-30 cells.

    Methods: OCCM-30 cells were treated with three concentrations of PGE2 (10, 100, and 1,000 ng/ml). Cell number, alkaline phosphatase (ALP) activity, and expression for mineralization-related genes were determined. Osteoprotegerin (OPG) and receptor activator of nuclear factor-kappa B (NF-κB) ligand (RANKL) expression were also examined by real-time polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA).

    Results: The addition of PGE2 at the highest dose used in this study suppressed cell proliferation of OCCM-30 cells. The expression of mineralization-related marker mRNA, such as type 1 collagen, ALP, bone sialoprotein (BSP), and osteocalcin (OCN), was constitutively detected in OCCM-30 cells. PGE2 dose dependently stimulated ALP activity and BSP-mRNA expression in OCCM-30 cells at day 3. Transcripts for OPG and RANKL and the protein level of OPG in culture media were upregulated with PGE2 stimulation.

    Conclusion: These results demonstrate that PGE2 suppressed cementoblast proliferation but stimulated ALP activity and the BSP-mRNA level, suggesting a role of PGE2 in controlling cementoblast differentiation, and further indicate that PGE2 modulates RANKL and OPG expression in cementoblasts; the increase of OPG secreted from cementoblasts with PGE2 stimulation may be essential to protect the root surface from resorption.