Distribution of FcγRIIa and FcγRIIIb Genotypes in Patients With Generalized Aggressive Periodontitis

• Autores: Rodrigo C.de Souza, Ana Paula V. Colombo
• Localización: Journal of periodontology, ISSN 0022-3492, Vol. 77, Nº. 7, 2006, págs. 1120-1128
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Distribution of FcγRIIa and FcγRIIIb Genotypes in Patients With Generalized Aggressive Periodontitis Rodrigo C. de Souza*† and Ana Paula V. Colombo‡ *Department of Periodontology, School of Dentistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

    †Department of Periodontology, Salgado de Oliveira University, Niteroi, Brazil.

    ‡Department of Medical Microbiology, Institute of Microbiology, Federal University of Rio de Janeiro.

    Correspondence: Dr. Ana Paula Vieira Colombo, Department of Medical Microbiology, Federal University of Rio de Janeiro, R. Gal. Dionísio, 60/804, 22271050 Rio de Janeiro, Brazil. Fax: 55-21-25077139; e-mail: apcolombo@micro.ufrj.br.

    Background: Polymorphisms in FcγR have been associated with different forms of periodontitis. This study determined the frequency of FcγRIIa and FcγRIIIb alleles/genotypes in patients with generalized aggressive periodontitis (GAgP).

    Methods: Thirty-one GAgP and 49 periodontally healthy Brazilian subjects participated in the study. Full-mouth periodontal examinations were carried out, and mouthwash samples were collected for human DNA isolation. FcγR genotyping was performed by polymerase chain reaction and hybridization with allele-specific oligonucleotide probes. Significant differences between groups were sought by Mann-Whitney, χ2, and Fisher exact tests and configural frequency analysis.

    Results: FcγRIIa-H131 (53.8%) and FcγRIIIb-NA1 (75%) were the most prevalent alleles in this sample population. A significant overrepresentation of FcγRIIIb-NA2 was observed in the GAgP group, whereas FcγRIIIb-NA1 was detected more often in healthy individuals (odds ratio, 32.5; 95% confidence interval [CI], 10.6 to 99.8; P <0.001). No significant differences in the distribution of the FcγRIIa genotypes were observed between the groups. The prevalence of FcγRIIIb-NA2/NA2 was higher in GAgP patients, whereas FcγRIIIb-NA1/NA1 was predominant in the healthy group (χ2 = 45.1; P <0.001). The combination of the genotypes FcγRIIIb-NA2/NA2 plus FcγRIIa-H/H131 was observed more frequently in GAgP subjects than expected from marginal frequencies (χ2 = 12.5; P <0.001).

    Conclusions: The data suggest that the FcγRIIIb-NA2 allele and/or FcγRIIIb-NA2/NA2 genotype and the composite genotype FcγRIIIb-NA2/NA2 plus FcγRIIa-H/H131 may be associated with GAgP, whereas FcγRIIIb-NA1 and/or FcγRIIIb-NA1/NA1 may be related to periodontal health in this sample of the Brazilian population.

    KEYWORDS: Fc gamma receptor, genotype, PCR, periodontitis, polymorphism Cited by Gwan Gyu Song and Young Ho Lee. (2013) Associations between FCGR2A rs1801274, FCGR3A rs396991, FCGR3B NA1/NA2 polymorphisms and periodontitis: a meta-analysis. Molecular Biology Reports 40, 4985-4993.

    Online publication date: 1-Aug-2013.

    CrossRef Scott R. Diehl, Chih-Hung Chou, Fengshen Kuo and Ching-Yu Huang. 2012. Genetic Factors and Periodontal Disease. Carranza's Clinical Periodontology, 271-284.

    CrossRef L. Chai, Y.-Q. Song and W. K. Leung. (2011) Genetic polymorphism studies in periodontitis and Fcγ receptors. Journal of Periodontal Research, no-no.

    Online publication date: 1-Nov-2011.

    CrossRef Niki L. Dimou, Georgios K. Nikolopoulos, Stavros J. Hamodrakas and Pantelis G. Bagos. (2010) Fc γ receptor polymorphisms and their association with periodontal disease: a meta-analysis. Journal of Clinical Periodontology 37:10.1111/cpe.2010.37.issue-3, 255-265.

    Online publication date: 1-Mar-2010.

    CrossRef Ya-Ping Ho, Yi-Hsin Yang, Kun-Yen Ho, Yi-Min Wu and Chi-Cheng Tsai. (2010) The association of Fc γ receptor IIIb genetic polymorphism and susceptibility to periodontitis in Taiwanese individuals. Journal of Clinical Periodontology 37:10.1111/cpe.2010.37.issue-2, 145-151.

    Online publication date: 1-Feb-2010.

    CrossRef T. Yokoyama, T. Kobayashi, K. Yamamoto, A. Yamagata, K. Oofusa and H. Yoshie. (2010) Proteomic profiling of human neutrophils in relation to immunoglobulin G Fc receptor IIIb polymorphism : Proteomics for Fcγ receptor IIIb polymorphism. Journal of Periodontal Research 45:6, 780.

    CrossRef José F. Siqueira Jr., Isabela N. Rôças, José C. Provenzano, Frederico K. Daibert, Marlei G. Silva and Kenio C. Lima. (2009) Relationship Between Fcγ Receptor and Interleukin-1 Gene Polymorphisms and Post-treatment Apical Periodontitis. Journal of Endodontics 35, 1186-1192.

    Online publication date: 1-Sep-2009.

    CrossRef R. P. M. Carvalho, J. S. Mesquita, A. Bonomo, P. X. Elsas and A. P. V. Colombo. (2009) Relationship of neutrophil phagocytosis and oxidative burst with the subgingival microbiota of generalized aggressive periodontitis. Oral Microbiology and Immunology 24, 124-132.

    Online publication date: 1-Apr-2009.

    CrossRef Edward J. Hollox, Jan-Christoph Detering and Tushna Dehnugara. (2009) An integrated approach for measuring copy number variation at the FCGR3 (CD16) locus. Human Mutation 30:10.1002/humu.v30:3, 477-484.

    Online publication date: 1-Mar-2009.

    CrossRef S. Li, M. H. Yang, C. A. Zeng, W. L. Wu, X. F. Huang, Y. Ji and J. Q. Zeng. (2008) Association of vitamin D receptor gene polymorphisms in Chinese patients with generalized aggressive periodontitis. Journal of Periodontal Research 43, 360-363.

    Online publication date: 1-Jun-2008.

    CrossRef Ali Gürkan, Gülnur Emingil, Buket Han Saygan, Gül Atilla, Serhat Çınarcık,, Timur Köse and Afig Berdeli. (2007) Matrix Metalloproteinase-2, -9, and -12 Gene Polymorphisms in Generalized Aggressive Periodontitis. Journal of Periodontology 78:12, 2338-2347.

    Online publication date: 1-Dec-2007.