Antibody Responses to Porphyromonas gingivalis Hemagglutinin A and Outer Membrane Protein in Chronic Periodontitis

• Autores: Yoshimitsu Abiko, Hiromasa Yoshie, Tetsuo Kobayashi, Susumu Kaneko, Tomoyuki Tahara, Mitsuo Hayakawa
• Localización: Journal of periodontology, ISSN 0022-3492, Vol. 77, Nº. 3, 2006, págs. 364-369
• Idioma: inglés
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• Resumen

  • Antibody Responses to Porphyromonas gingivalis Hemagglutinin A and Outer Membrane Protein in Chronic Periodontitis Tetsuo Kobayashi,*† Susumu Kaneko,* Tomoyuki Tahara,‡ Mitsuo Hayakawa,§ Yoshimitsu Abiko,§ and Hiromasa Yoshie* *Division of Periodontology, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.

    †General Dentistry and Clinical Education Unit, Niigata University Medical and Dental Hospital, Niigata, Japan.

    ‡Pharmaceutical Research Laboratory, Kirin Brewery, Gunma, Japan.

    §Department of Biochemistry and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Chiba, Japan.

    Correspondence: Dr. Tetsuo Kobayashi, General Dentistry and Clinical Education Unit, Niigata University Medical and Dental Hospital, 2-5274 Gakkocho-dori, Niigata 951-8514, Japan. Fax: 81-25-227-0991; e-mail: kotetsuo@dent.niigata-u.ac.jp.

    Background: Hemagglutinin and outer membrane protein (OMP) are major virulence factors associated with colonization of Porphyromonas gingivalis in the gingival crevice. The genes for the 200-kDa antigenic protein (200-kDa AP) and 40-kDa OMP of P. gingivalis have been successfully cloned. Additionally, the 200-kDa AP gene has been shown to constitute the hemagglutinin A (hagA) gene of P. gingivalis. Therefore, this study was constructed to evaluate the distributions and serum levels of immnoglobulin G (IgG) antibodies specific for 200-kDa AP and 40-kDa OMP in periodontitis patients.

    Methods: Fifty patients with chronic periodontitis and 59 controls without periodontal destruction were enrolled in this study. We cloned the genes for 200-kDa AP and 40-kDa OMP from P. gingivalis and constructed the purified recombinant proteins. Serum levels of IgG subclass antibodies specific for both recombinant 200-kDa and 40-kDa OMP were determined in patients and controls by an enzyme-linked immunosorbent assay (ELISA).

    Results: The serum IgG subclass distribution for patients and controls was IgG1 > IgG4 > IgG2 > IgG3 in the anti-200-kDa AP response, which was almost identical to that in the anti-40-kDa OMP response. The patient group showed significantly higher serum IgG responses to the 40-kDa OMP than the control group (P <0.01). In contrast, IgG subclass responses to the 200-kDa AP were not different between the patients and controls. Serum levels of antibodies reactive with both 200-kDa and 40-kDa proteins did not have a significant association with mean probing depth.

    Conclusion: These results suggested that serum IgG responses against P. gingivalis OMP rather than the hagA may be more active in chronic periodontitis.

    KEYWORDS: Antibody response, chronic periodontitis, hemagglutinin A, outer membrane protein, Porphyromonas gingivalis Cited by Satoshi Yuzawa, Tomoko Kurita-Ochiai, Tomomi Hashizume, Ryoki Kobayashi, Yoshimitsu Abiko and Masafumi Yamamoto. (2011) Sublingual vaccination with fusion protein consisting of the functional domain of hemagglutinin A of Porphyromonas gingivalis and Escherichia coli maltose-binding protein elicits protective immunity in the oral cavity. FEMS Immunology & Medical Microbiology, n/a-n/a.

    Online publication date: 1-Dec-2011.

    CrossRef N. Sugita, R. Iwanaga, T. Kobayashi and H. Yoshie. (2011) Association of the FcγRIIB-nt645+25A/G polymorphism with the expression level of the FcγRIIb receptor, the antibody response to Porphyromonas gingivalis and the severity of periodontitis. Journal of Periodontal Research, no-no.

    Online publication date: 1-Sep-2011.

    CrossRef Jun SASAHARA, Akira KIKUCHI, Koichi TAKAKUWA, Noriko SUGITA, Yoshimitsu ABIKO, Hiromasa YOSHIE and Kenichi TANAKA. (2009) Antibody responses to Porphyromonas gingivalis outer membrane protein in the first trimester. Australian and New Zealand Journal of Obstetrics and Gynaecology 49:10.1111/ajo.2009.49.issue-2, 137-141.

    Online publication date: 1-Apr-2009.

    CrossRef Y. Honma, N. Sugita, T. Kobayashi, Y. Abiko and H. Yoshie. (2008) Lower antibody response to Porphyromonas gingivalis associated with immunoglobulin G Fcγ receptor II B polymorphism. Journal of Periodontal Research 43:10.1111/jre.2008.43.issue-6, 706-711.

    Online publication date: 1-Dec-2008.

    CrossRef Hamdan S. Al-Ghamdi and Sukumaran Anil. (2007) Serum Antibody Levels in Smoker and Non-Smoker Saudi Subjects With Chronic Periodontitis. Journal of Periodontology 78:6, 1043-1050.

    Online publication date: 1-Jun-2007.