Resumen de Periodontitis Deteriorates Metabolic Control in Type 2 Diabetic Goto-Kakizaki Rats
Kaj Stoltze, Palle Holmstrup, Carla C. Pontes Andersen, Karsten Buschard, Allan Flyvbjerg
Periodontitis Deteriorates Metabolic Control in Type 2 Diabetic Goto-Kakizaki Rats Carla C. Pontes Andersen,* Karsten Buschard,† Allan Flyvbjerg,‡ Kaj Stoltze,* and Palle Holmstrup* *Department of Periodontology, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.
†Bartholin Instituttet, Rigshospitalet, Copenhagen, Denmark.
‡The Medical Research Laboratories, Clinical Institute, Aarhus University Hospital, Aarhus, Denmark.
Correspondence: Dr. Carla C. Pontes Andersen, Department of Periodontology, School of Dentistry, University of Copenhagen, Noerre Alle 20, DK-2200 Copenhagen, Denmark. Fax: 45-35-32-66-99; e-mail: cpo@odont.ku.dk.
Background: Epidemiologic and clinical studies have indicated that periodontal disease (PD) may cause disturbances in general health and even affect diabetes. The aim of this study was to gain knowledge on the effect of PD on diabetes metabolic control in a new model for type 2 diabetes–associated PD (i.e., the Goto-Kakizaki [GK] rat).
Methods: GK rats represented the type 2 diabetes group and were allocated into two groups: diabetes or diabetes + PD group; Wistar rats represented the non-diabetes group and were divided into non-diabetes + PD and non-diabetes groups. PD was induced by placing ligatures around second maxillary molars, and the animals were followed for 6 weeks. Serum insulin, glucose, and free fatty acid levels were evaluated; interleukin (IL)-1β, IL-6, and tumor necrosis factor-α were measured in adipose tissue supernatant; glucose tolerance and insulin resistance were calculated. Further, alveolar bone destruction was estimated morphometrically and radiographically.
Results: Rats with diabetes + PD became almost 30% more glucose intolerant (P <0.01) and presented a 25% increase in IL-1β in adipose tissue (P <0.05) compared to rats from the diabetes group. Moreover, PD associated with diabetes resulted in more alveolar bone destruction in comparison to PD in the absence of diabetes (P <0.01).
Conclusions: Our results indicated that PD deteriorates metabolic control in diabetes, which emphasizes that PD may play a significant role for the course of diabetes. The GK rat can represent a suitable model for further studies on the association between PD and diabetes.
KEYWORDS: Animal models, diabetes, periodontitis, rats Cited by Cüneyt Asım Aral, Servet Kesim, Henry Greenwell, Mehmet Kara, Aysun Çetin and Birkan Yakan. (2015) Alveolar Bone Protective and Hypoglycemic Effects of Systemic Propolis Treatment in Experimental Periodontitis and Diabetes Mellitus. Journal of Medicinal Food 18, 195-201.
Online publication date: 1-Feb-2015.
CrossRef Fawad Javed, Hameeda Bashir Ahmed, Abid Mehmood, Crawford Bain and Georgios E. Romanos. (2014) Effect of nonsurgical periodontal therapy (with or without oral doxycycline delivery) on glycemic status and clinical periodontal parameters in patients with prediabetes: a short-term longitudinal randomized case–control study. Clinical Oral Investigations 18, 1963-1968.
Online publication date: 1-Nov-2014.
CrossRef Daisy J. Shirakashi, Rosana P. Leal, Natalia H. Colombo, Fernando Y. Chiba, Cléa A.S. Garbin, Elerson G. Jardim Jr., Cristina Antoniali and Doris H. Sumida. (2013) Maternal Periodontal Disease in Rats Decreases Insulin Sensitivity and Insulin Signaling in Adult Offspring. Journal of Periodontology 84:3, 407-414.
Online publication date: 1-Mar-2013.
Abstract | Full Text | PDF (751 KB) | PDF Plus (610 KB) Ryota Torii, Noritoshi Yamamoto, Masakazu Hasegawa, Seeta Kato, Ryoko Kawai, Waka Yoshida, Yoshihiko Sugita, Emiko Sato, Katsutoshi Kubo and Hatsuhiko Maeda. (2013) An Investigation of Osteogenesis on Titanium Surfaces using a Type 2 Diabetes Rat Model. Journal of Hard Tissue Biology 22, 439-444.
Online publication date: 1-Jan-2013.
CrossRef Selin P. Özdemir, Bülent Kurtiş, Gülay Tüter, Şeyma Bozkurt, Sibel Elif Gültekin, Burcu Sengüven, Keiko Watanabe and Sevtap Aydın. (2012) Effects of Low-Dose Doxycycline and Bisphosphonate Clodronate on Alveolar Bone Loss and Gingival Levels of Matrix Metalloproteinase-9 and Interleukin-1β in Rats With Diabetes: A Histomorphometric and Immunohistochemical Study. Journal of Periodontology 83:9, 1172-1182.
Online publication date: 1-Sep-2012.
Abstract | Full Text | PDF (3753 KB) | PDF Plus (1044 KB) Natalia H. Colombo, Daisy J. Shirakashi, Fernando Y. Chiba, Maria Sara de Lima Coutinho, Edilson Ervolino, Cléa Adas Saliba Garbin, Ubiratan Fabres Machado and Doris H. Sumida. (2012) Periodontal Disease Decreases Insulin Sensitivity and Insulin Signaling. Journal of Periodontology 83:7, 864-870.
Online publication date: 1-Jul-2012.
Abstract | Full Text | PDF (1235 KB) | PDF Plus (618 KB) Lei Chen, Gang Luo, Dongying Xuan, Bihong Wei, Fang Liu, Jing Li and Jincai Zhang. (2012) Effects of Non-Surgical Periodontal Treatment on Clinical Response, Serum Inflammatory Parameters, and Metabolic Control in Patients With Type 2 Diabetes: A Randomized Study. Journal of Periodontology 83:4, 435-443.
Online publication date: 1-Apr-2012.
Abstract | Full Text | PDF (524 KB) | PDF Plus (544 KB) Björn Klinge and Jörgen Jönsson. 2011.
CrossRef K. Watanabe, T. Iizuka, A. Adeleke, L. Pham, A. E. Shlimon, M. Yasin, P. Horvath and T. G. Unterman. (2010) Involvement of toll-like receptor 4 in alveolar bone loss and glucose homeostasis in experimental periodontitis. Journal of Periodontal Research, no-no.
Online publication date: 1-Sep-2010.
CrossRef Guang Yu, Yang Yu, Yi Ning Li and Rong Shu. (2010) Effect of periodontitis on susceptibility to atrial fibrillation in an animal model☆. Journal of Electrocardiology 43, 359-366.
Online publication date: 1-Jul-2010.
CrossRef Johanne Kongstad, Ulla A. Hvidtfeldt, Morten Grønbæk, Kaj Stoltze and Palle Holmstrup. (2009) The Relationship Between Body Mass Index and Periodontitis in the Copenhagen City Heart Study. Journal of Periodontology 80:8, 1246-1253.
Online publication date: 1-Aug-2009.
Abstract | Full Text | PDF (485 KB) | PDF Plus (507 KB) Keiko Watanabe, Benjamin J. Petro, Alan E. Shlimon and Terry G. Unterman. (2008) Effect of Periodontitis on Insulin Resistance and the Onset of Type 2 Diabetes Mellitus in Zucker Diabetic Fatty Rats. Journal of Periodontology 79:7, 1208-1216.
Online publication date: 1-Jul-2008.
Abstract | Full Text | PDF (933 KB) | PDF Plus (609 KB) A. Schulze, M. Schönauer and M. Busse. (2007) Sudden Improvement of Insulin Sensitivity Related to an Endodontic Treatment. Journal of Periodontology 78:12, 2380-2384.
Online publication date: 1-Dec-2007.
Abstract | Full Text | PDF (1038 KB) | PDF Plus (209 KB) Henry Völzke, Christian Schwahn, Marcus Dörr, Nicole Aumann, Stephan B Felix, Ulrich John, Rainer Rettig, Martina Dören, Reiner Biffar and Thomas Kocher. (2007) Inverse association between number of teeth and left ventricular mass in women. Journal of Hypertension 25, 2035-2043.
Online publication date: 1-Oct-2007.
CrossRef Carla C. Pontes Andersen, Allan Flyvbjerg, Karsten Buschard and Palle Holmstrup. (2007) Periodontitis Is Associated With Aggravation of Prediabetes in Zucker Fatty Rats. Journal of Periodontology 78:3, 559-565.
Online publication date: 1-Mar-2007.
