Resumen de Inhibition of Proinflammatory Activities of Major Periodontal Pathogens by Aqueous Extracts From Elder Flower (Sambucus nigra)
Frank A. Scannapieco, George Hajishengallis, Evlambia Harokopakis, Mohamad H. Albzreh, Elaine M. Haase
Inhibition of Proinflammatory Activities of Major Periodontal Pathogens by Aqueous Extracts From Elder Flower (Sambucus nigra) Evlambia Harokopakis,* Mohamad H. Albzreh,† Elaine M. Haase,‡ Frank A. Scannapieco,‡ and George Hajishengallis§ *Department of Pedodontics, School of Dentistry, Center of Excellence in Oral and Craniofacial Biology, Louisiana State University Health Sciences Center, New Orleans, LA.
†Department of Periodontics, School of Dentistry, Center of Excellence in Oral and Craniofacial Biology, Louisiana State University Health Sciences Center.
‡Department of Oral Biology, School of Dental Medicine, State University of New York, Buffalo, NY.
§Currently, Department of Periodontics and Endodontics, University of Louisville School of Dentistry, Louisville, KY; previously, Department of Microbiology and Immunology, School of Dentistry, Center of Excellence in Oral and Craniofacial Biology, Louisiana State University Health Sciences Center.
Correspondence: Dr. George Hajishengallis, Department of Periodontics and Endodontics, University of Louisville School of Dentistry, 501 South Preston St., Louisville, KY 40292. Fax. 502/852-4052; e-mail: g0haji01@louisville.edu.
Background: Prolonged induction of excessive levels of inflammatory mediators contributes to the pathogenesis of chronic disease states, such as periodontitis. It is thus important to develop safe and effective anti-inflammatory strategies for therapeutic reasons. In this study, we determined the ability of aqueous extracts from elder flower (Sambucus nigra) to inhibit the proinflammatory activity of major virulence factors from the periodontal pathogens Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans.
Methods: Monocytes/macrophages or neutrophils were incubated with whole cells of P. gingivalis, A. actinomycetemcomitans, or purified components thereof (lipopolysaccharide and fimbriae) in the absence or presence of elder flower extract and were assayed for cytokine production, integrin activation, or induction of the oxidative burst.
Results: The elder flower extract was found to potently inhibit all proinflammatory activities tested. Investigation of the underlying mechanisms revealed that the anti-inflammatory extract inhibited activation of the nuclear transcription factor κB and of phosphatidylinositol 3-kinase.
Conclusion: The elder flower extract displays useful anti-inflammatory properties that could be exploited therapeutically for the control of inflammation in human periodontitis.
KEYWORDS: Actinobacillus actinomycetemcomitans, cytokines, inflammation, nuclear factor-kappa B, Porphyromonas gingivalis, Sambucus nigra Cited by Liat Chaushu, Miron Weinreb, Ilan Beitlitum, Ofer Moses and Carlos E. Nemcovsky. (2015) Evaluation of a topical herbal patch for soft tissue wound healing: an animal study. Journal of Clinical Periodontology 42:10.1111/jcpe.2015.42.issue-3, 288-293.
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