Collagenases in Gingival Crevicular Fluid in Type 1 Diabetes Mellitus
- Autores: Arzu Beklen, Yrjö T. Konttinen, Bedia Safkan-Seppälä, Timo Sorsa, Taina Tervahartiala
- Localización: Journal of periodontology, ISSN 0022-3492, Vol. 77, Nº. 2, 2006, págs. 189-194
- Idioma: inglés
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Resumen
Collagenases in Gingival Crevicular Fluid in Type 1 Diabetes Mellitus Bedia Safkan-Seppälä,* Timo Sorsa,* Taina Tervahartiala,* Arzu Beklen,†‡§ and Yrjö T. Konttinen†‖¶ *Institute of Dentistry, University of Helsinki, Helsinki, Finland.
†Department of Medicine/Invärtes Medicin, Helsinki University Hospital, Helsinki, Finland.
‡Institute of Biomedicine/Anatomy, University of Helsinki.
§Medico-Social Centre, Dental Clinic, Bogazici University, Istanbul, Turkey.
‖ORTON Orthopaedic Hospital of the Invalid Foundation, Helsinki, Finland.
¶COXA Joint Replacement Hospital, Tampere, Finland.
Correspondence: Prof. Yrjö T. Konttinen, Biomedicum, P.O. Box 700 (Haartmaninkatu 8), Helsinki, FIN-00029 HUS Finland. Fax: 358-9-191-25218; e-mail: yrjo.konttinen@helsinki.fi.
Background: Studies have demonstrated that high levels of collagenase activity in gingival crevicular fluid (GCF) are associated with degradation of periodontal tissues in progressive periodontitis compared to periodontally healthy tissues. Because the activation of collagenases is an important issue in periodontitis, we have studied the activation of collagenase in gingival crevicular fluid samples of diabetic patients.
Methods: Collagenase activity was studied in human gingival crevicular fluids. Twenty-two poorly controlled diabetic patients (e.g., blood glucose: 11.0 ± 0.7 mmol/l; hemoglobin A1c [HbA1c]: 9.6% ± 0.3%) and five well-controlled diabetic patients were compared to six chronic periodontitis subjects and five healthy controls. Collagenase activity against type I collagen was measured using sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis quantitated by laser densitometry.
Results: The poorly controlled diabetic patients had more alveolar bone loss than the well-controlled diabetic subjects and controls (P <0.001; t test). The activity of collagenases in GCF in poorly controlled diabetic patients was similar to that seen in chronic periodontitis subjects (P >0.05) but higher than in healthy controls (P <0.01; t test), whereas there was no difference between the well-controlled diabetic subjects and systemically healthy controls (P >0.05; t test).
Conclusion: Poorly controlled diabetes is strongly related to periodontal tissue destruction, and collagenases in GCF may mediate and reflect this effect.
KEYWORDS: Collagenase, gingival crevicular fluid, insulin-dependent diabetes mellitus, periodontitis Cited by Ilana Perelshtein, Elena Ruderman, Antonio Francesko, Margarida M. Fernandes, Tzanko Tzanov and Aharon Gedanken. (2014) Tannic acid NPs – Synthesis and immobilization onto a solid surface in a one-step process and their antibacterial and anti-inflammatory properties. Ultrasonics Sonochemistry 21, 1916-1920.
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Abstract | PDF (701 KB) | PDF Plus (883 KB) John J. Taylor, Philip M. Preshaw and Evanthia Lalla. (2013) A review of the evidence for pathogenic mechanisms that may link periodontitis and diabetes. Journal of Clinical Periodontology 40, S113-S134.
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