Resumen de Apoptosis and Expression of Caspase-3 in Cyclosporin-Induced Gingival Overgrowth
Apoptosis and Expression of Caspase-3 in Cyclosporin-Induced Gingival Overgrowth Sule Bulut* and B. Handan Özdemir† *Department of Periodontology, Faculty of Dentistry, University of Baskent, Ankara, Turkey.
†Department of Pathology, Faculty of Medicine, University of Baskent.
Correspondence: Dr. Sule Bulut, Department of Periodontology, Faculty of Dentistry, University of Baskent, 11 Sok, No. 26, 06490, Bahçelievler, Ankara, Turkey. Fax: 90-312-215-29-62; e-mail: suleb@baskent.edu.tr.
Background: The pathogenesis of epithelial thickening in gingival overgrowth remains obscure. Apoptosis plays an important role in maintaining tissue hemostasis. The aim of the present study was to investigate apoptosis via immunohistochemical analyses in cyclosporin A–induced gingival overgrowth tissue samples to determine whether these processes play a role in the pathogenesis of gingival overgrowth.
Methods: Gingival biopsies (one per person) were harvested from 22 renal transplant recipients (eight men and 14 women; mean age, 36.4 ± 13.3 years) who had been diagnosed with cyclosporin A–induced gingival enlargement and from 12 systemically healthy persons (seven men and five women; mean age, 27.0 ± 16.0 years) with plaque-induced gingivitis. Distributions of caspase-3 and apoptosis were determined immunologically.
Results: Significant differences were found with regard to caspase-3 levels and the extent of apoptosis between the cyclosporin A group and the control group. Plaque index, gingival index, and probing depths were significantly lower in the control group.
Conclusion: The extent of keratinocyte apoptosis and decreased levels of caspase-3 may be an important factor affecting the gingiva of kidney transplant recipients with cyclosporin A-induced gingival overgrowth.
KEYWORDS: Apoptosis, cyclosporin A, gingival overgrowth, keratinocyte, pathogenesis Cited by Su Ma, Peihong Liu, Yanwu Li, Lin Hou, Li Chen and Chunlin Qin. (2014) Cyclosporine A Inhibits Apoptosis of Rat Gingival Epithelium. Journal of Periodontology 85:8, 1126-1134.
Online publication date: 1-Aug-2014.
Abstract | Full Text | PDF (3138 KB) | PDF Plus (914 KB) Reiri Takeuchi, Hiroko Matsumoto, Yoshiaki Akimoto and Akira Fujii. (2011) Reduction in lipopolysaccharide-induced apoptosis of fibroblasts obtained from a patient with gingival overgrowth during nifedipine-treatment. Archives of Oral Biology 56, 1073-1080.
Online publication date: 1-Oct-2011.
CrossRef H.-P. Tu, Y.-T. Chen, H.-C. Chiu, Y.-T. Chin, S.-M. Huang, L.-C. Cheng, E. Fu and C.-Y. Chiang. (2009) Cyclosporine A enhances apoptosis in gingival keratinocytes of rats and in OECM1 cells via the mitochondrial pathway. Journal of Periodontal Research 44:10.1111/jre.2009.44.issue-6, 767-775.
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CrossRef Naohiro Nakasone, Takehiko Kubota, Chika Hoshino, Kaname Nohno, Manami Itagaki, Taro Shimizu and Hiromasa Yoshie. (2009) Differential gene and protein expression of tissue inhibitors of metalloproteinases (TIMP)-3 and TIMP-4 in gingival tissues from drug induced gingival overgrowth. Archives of Oral Biology 54, 634-641.
Online publication date: 1-Jul-2009.
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