Resumen de Subgingival Human Cytomegalovirus Correlates With Increased Clinical Periodontal Parameters and Bacterial Coinfection in Periodontitis
Beatriz Parra, Gustavo Adolfo Contreras García, Javier Enrique Botero, Adriana Jaramillo
Subgingival Human Cytomegalovirus Correlates With Increased Clinical Periodontal Parameters and Bacterial Coinfection in Periodontitis Javier Enrique Botero,*† Beatriz Parra,*‡ Adriana Jaramillo,* and Adolfo Contreras*† *Periodontal Medicine Group, University of Valle, Cali, Colombia.
†Periodontics Department, School of Dentistry, University of Valle.
‡Emerging Virus Research Group (VIREM), Microbiology Department, University of Valle.
Correspondence: Dr. Javier Enrique Botero, School of Odontology, University of Valle, Calle 4B#36-00 San Fernando, Cali, Colombia. E-mail: drjavo@yahoo.com.
Background: Viruses from the Herpesviridae family may be implicated in the pathogenesis of periodontal disease. The aim of this investigation was to compare the subgingival frequency of human cytomegalovirus (HCMV) in subjects affected by periodontitis to periodontally healthy subjects and to assess the correlation of HCMV with periodontal clinical parameters and periodontopathic bacteria.
Methods: Thirty subjects with periodontitis (20 with chronic periodontitis and 10 with aggressive periodontitis) were included in the study. A group of 22 periodontally healthy individuals served as controls. Clinical periodontal parameters of probing depth (PD) and clinical attachment level (CAL) were recorded using a computerized periodontal probe. Subgingival plaque samples were processed for viral identification by nested polymerase chain reaction and bacterial identification by culture. Clinical periodontal parameters, frequency of detection of HCMV, and microbial composition were compared between the groups using the two-tailed Student t, χ2, and Mann-Whitney tests. Logistic and linear regression analyses were performed to measure the association between virus-bacterial coinfection and clinical parameters (P ≤0.05).
Results: HCMV detection was more prevalent (P ≤0.05) in periodontally diseased subjects compared to healthy ones. Furthermore, in all groups, PD and CAL were increased in HCMV-positive sites. In the periodontitis groups, higher frequencies and levels of specific periodontopathic bacteria were detected in HCMV-positive sites.
Conclusions: HCMV detection in periodontal pockets was associated with higher levels of periodontopathic bacteria and increased PD and CAL at sampled sites. HCMV/bacteria coinfection may be an important factor in periodontal destruction.
KEYWORDS: Cytomegalovirus, infection, periodontitis, Porphyromonas gingivalis Cited by Adolfo Contreras, Sandra M. Moreno, Adriana Jaramillo, Melissa Pelaez, Andres Duque, Javier E. Botero and Jørgen Slots. (2015) Periodontal microbiology in Latin America. Periodontology 2000 67:10.1111/prd.2015.67.issue-1, 58-86.
Online publication date: 1-Feb-2015.
CrossRef Eija Könönen and Hans-Peter Müller. (2014) Microbiology of aggressive periodontitis. Periodontology 2000 65:10.1111/prd.2014.65.issue-1, 46-78.
Online publication date: 1-Jun-2014.
CrossRef Tung Nguyen-Hieu. (2013) Microbial sampling process can change results of microbiological analysis in periodontitis diagnosis. A minireview. Journal of Investigative and Clinical Dentistry 4:10.1111/jicd.2013.4.issue-3, 151-159.
Online publication date: 1-Aug-2013.
CrossRef Gary C. Armitage. (2013) Learned and unlearned concepts in periodontal diagnostics: a 50-year perspective. Periodontology 2000 62:10.1111/prd.2013.62.issue-1, 20-36.
Online publication date: 1-Jun-2013.
CrossRef Carlo Bertoldi, Chiara Pellacani, Michele Lalla, Ugo Consolo, Marcello Pinti, Pierpaolo Cortellini and Andrea Cossarizza. (2012) Herpes Simplex I virus impairs regenerative outcomes of periodontal regenerative therapy in intrabony defects. A pilot study. Journal of Clinical Periodontology 39, 385-392.
Online publication date: 1-Apr-2012.
CrossRef S Jankovic, Z Aleksic, B Dimitrijevic, V Lekovic, I Milinkovic and B Kenney. (2011) Correlation between different genotypes of human cytomegalovirus and Epstein-Barr virus and peri-implant tissue status. Australian Dental Journal, no-no.
Online publication date: 1-Sep-2011.
CrossRef S. Jankovic, Z. Aleksic, B. Dimitrijevic, V. Lekovic, P. Camargo and B. Kenney. (2011) Prevalence of human cytomegalovirus and Epstein-Barr virus in subgingival plaque at peri-implantitis, mucositis and healthy sites. A pilot study. International Journal of Oral and Maxillofacial Surgery 40, 271-276.
Online publication date: 1-Mar-2011.
CrossRef Birgitta Söder, Maha Yakob, Jukka H. Meurman, Leif C. Andersson, Björn Klinge and Per-Östen Söder. (2010) Periodontal disease may associate with breast cancer. Breast Cancer Research and Treatment.
Online publication date: 20-Oct-2010.
CrossRef Jørgen Slots. (2010) Human viruses in periodontitis. Periodontology 2000 53:10.1111/prd.2010.53.issue-1, 89-110.
Online publication date: 1-Jun-2010.
CrossRef M. Chalabi, F. Rezaie, S. Moghim, A. Mogharehabed, M. Rezaei and B. Mehraban. (2010) Periodontopathic bacteria and herpesviruses in chronic periodontitis. Molecular Oral Microbiology 25:10.1111/omi.2010.25.issue-3, 236-240.
Online publication date: 1-Jun-2010.
CrossRef Jorge Hernán Ramirez, Roger Arce and Adolfo Contreras. (2010) Why Must Physicians Know About Oral Diseases?. Teaching and Learning in Medicine 22, 148-155.
Online publication date: 2-Apr-2010.
CrossRef Jørgen Slots. (2010) Herpesviralâbacterial interactions in periodontal diseases. Periodontology 2000 52:10.1111/prd.2009.52.issue-1, 117-140.
Online publication date: 1-Feb-2010.
CrossRef Luigi Nibali, Claire Atkinson, Paul Griffiths, Ulpee Darbar, Thanasak Rakmanee, Jeanie Suvan and Nikos Donos. (2009) Low prevalence of subgingival viruses in periodontitis patients. Journal of Clinical Periodontology 36:10.1111/cpe.2009.36.issue-11, 928-932.
Online publication date: 1-Nov-2009.
CrossRef DR Dawson III, C Wang, RJ Danaher, Y Lin, RJ Kryscio, RJ Jacob and CS Miller. (2009) Salivary levels of Epstein-Barr virus DNA correlate with subgingival levels, not severity of periodontitis. Oral Diseases 15:10.1111/odi.2009.15.issue-8, 554-559.
Online publication date: 1-Nov-2009.
CrossRef A. Contreras and J. Ramírez. (2009) Relación entre Periodontitis y Enfermedad Cardiovascular. Revista Clínica de Periodoncia, Implantología y Rehabilitación Oral 2, 91-97.
Online publication date: 1-Aug-2009.
CrossRef Dolphus R. Dawson , III, Chunmei Wang, Robert J. Danaher, Yushun Lin, Richard J. Kryscio, Robert J. Jacob and Craig S. Miller. (2009) Real-Time Polymerase Chain Reaction to Determine the Prevalence and Copy Number of Epstein-Barr Virus and Cytomegalovirus DNA in Subgingival Plaque at Individual Healthy and Periodontal Disease Sites. Journal of Periodontology 80:7, 1133-1140.
Online publication date: 1-Jul-2009.
Abstract | Full Text | PDF (577 KB) | PDF Plus (554 KB) Ana Vitória Imbronito, Osmar Shizuo Okuda, Nivea Maria de Freitas, Roberto Fraga Moreira Lotufo and Fabio Daumas Nunes. (2008) Detection of Herpesviruses and Periodontal Pathogens in Subgingival Plaque of Patients With Chronic Periodontitis, Generalized Aggressive Periodontitis, or Gingivitis. Journal of Periodontology 79:12, 2313-2321.
Online publication date: 1-Dec-2008.
Abstract | Full Text | PDF (491 KB) | PDF Plus (536 KB) D COMBS, E REILLY, D DAWSONIII, S AVDIUSHKO, R DANAHER and C MILLER. (2008) Detection of human cytomegalovirus in dental plaque from individual periodontal sites by real-time polymerase chain reaction. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology 106, 840-844.
Online publication date: 1-Dec-2008.
CrossRef Antonella Rotola, Enrico Cassai, Roberto Farina, Elisabetta Caselli, Valentina Gentili, Tiziana Lazzarotto and Leonardo Trombelli. (2008) Human herpesvirus 7, Epstein-Barr virus and human cytomegalovirus in periodontal tissues of periodontally diseased and healthy subjects. Journal of Clinical Periodontology 35:10.1111/cpe.2008.35.issue-10, 831-837.
Online publication date: 1-Oct-2008.
