In Vitro Biologic Response of Human Bone Marrow Stromal Cells to Enamel Matrix Derivative

• Autores: Angelo Di Feo, Adriana Oliva, Luigi Guida, Marco Annunziata, Francesco Carinci, Irene Passaro
• Localización: Journal of periodontology, ISSN 0022-3492, Vol. 78, Nº. 11, 2007, págs. 2190-2196
• Idioma: inglés
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• Resumen

  • In Vitro Biologic Response of Human Bone Marrow Stromal Cells to Enamel Matrix Derivative Luigi Guida,* Marco Annunziata,* Francesco Carinci,† Angelo Di Feo,‡ Irene Passaro,‡ and Adriana Oliva‡ *Department of Odontostomatological, Orthodontic and Surgical Disciplines, Second University of Naples, Naples, Italy.

    †Department of Maxillofacial Surgery, University of Ferrara, Ferrara, Italy.

    ‡Department of Biochemistry and Biophysics “F. Cedrangolo,” Second University of Naples.

    Correspondence: Prof. Adriana Oliva, Department of Biochemistry and Biophysics “F. Cedrangolo,” Second University of Naples, Via Luigi De Crecchio 7, 80138 Naples, Italy. Fax: 39-081-5667608; e-mail: adriana.oliva@unina2.it.

    Background: In vitro investigations suggest that enamel matrix derivative (EMD) may affect the biologic response of periodontal-related cells, including osteoblasts and their precursors, the bone marrow stromal cells (BMSCs), which could play a crucial role in the regenerative process. In this study, we investigated the effects of EMD on human BMSCs.

    Methods: Primary cultures of BMSCs were obtained from bone marrow samples of healthy donors. Cell proliferation and osteogenic marker expression in response to serial dilutions of EMD (12.5, 25, and 50 μg/ml) were assessed. Cell growth was measured by 3H-thymidine incorporation and type I collagen synthesis by immunoblotting. Alkaline phosphatase (AP)-specific activity in the early phase (7 days), in vitro mineralization by von Kossa staining and calcium quantification, and osteocalcin levels at prolonged times (3 weeks) also were evaluated.

    Results: EMD stimulated BMSC growth in a dose-dependent manner. When EMD 50 μg/ml was followed over time, the highest proliferative effect was evident at 24 hours (3.4-fold of the control). Type I collagen level was significantly lower than the control after a 7-day incubation with EMD 50 μg/ml. AP activity was reduced in a dose-dependent manner down to 55% of the control. Also, the extracellular matrix mineralization decreased in EMD-treated cells with respect to the control, whereas only a slight, not significant, decrease in osteocalcin levels was found.

    Conclusions: EMD significantly increased BMSC growth and simultaneously decreased their osteogenic differentiation. The clinical efficacy of EMD in regenerating periodontal tissues can be attributed, in part, to the biologic effects exerted on the bone marrow stromal component of resident cells.

    KEYWORDS: Bone marrow, cell differentiation, cell proliferation, enamel matrix proteins, stromal cells Cited by Higinio Arzate, Margarita Zeichner-David and Gabriela Mercado-Celis. (2015) Cementum proteins: role in cementogenesis, biomineralization, periodontium formation and regeneration. Periodontology 2000 67:10.1111/prd.2015.67.issue-1, 211-233.

    Online publication date: 1-Feb-2015.

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