Platelet-Rich Plasma: Growth Factors and Pro- and Anti-Inflammatory Properties

• Autores: Hongsheng Liu, Thomas E. Van Dyke, Hesham El-Sharkawy, Alpdogan Kantarci, Hatice Hasturk, Mohammad Alshahat
• Localización: Journal of periodontology, ISSN 0022-3492, Vol. 78, Nº. 4, 2007, págs. 661-669
• Idioma: inglés
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• Resumen

  • Platelet-Rich Plasma: Growth Factors and Pro- and Anti-Inflammatory Properties Hesham El-Sharkawy,*† Alpdogan Kantarci,* Jennifer Deady,* Hatice Hasturk,* Hongsheng Liu,* Mohammad Alshahat,† and Thomas E. Van Dyke* *Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, MA.

    †Department of Periodontology and Oral Medicine, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.

    Correspondence: Dr. Thomas E. Van Dyke, Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, 100 E. Newton St. G-107, Boston, MA 02118. Fax: 617/638-4799; e-mail: tvandyke@bu.edu.

    Background: Platelet-rich plasma (PRP) promotes regeneration of bone, presumably through the action of concentrated growth factors. However, it is not clear how PRP affects the inflammatory response. The purpose of this study was to analyze the growth factors in PRP and to study the effects of PRP on monocyte cytokine release and lipoxin A4 (LXA4) generation.

    Methods: PRP was prepared from healthy donors. Platelet-derived growth factor (PDGF)-AB, PDGF-BB, transforming growth factor-β1, insulin-like growth factor-I, fibroblast growth factor-basic (FGF-b), epidermal growth factor (EGF), vascular endothelial growth factor, interleukin-12 (p40/70), and regulated on activation, normal T-cell expressed and secreted (RANTES) levels were evaluated by enzyme-linked immunosorbent assay and bead-based multiplexing. Peripheral blood monocytes were isolated and cultured with or without PRP. Cytokine, chemokine, and LXA4 levels as well as monocyte chemotactic migration were analyzed.

    Results: Growth factors were increased significantly in PRP compared to whole blood (WB) and platelet-poor plasma. Monocyte chemotactic protein-1 (MCP-1) was suppressed significantly by PRP, whereas RANTES was increased significantly in monocyte cultures. LXA4 levels were significantly higher in PRP compared to WB. PRP stimulated monocyte chemotaxis in a dose-dependent fashion, whereas RANTES, in part, was responsible for PRP-mediated monocyte migration.

    Conclusions: PRP is a rich source of growth factors and promoted significant changes in monocyte-mediated proinflammatory cytokine/chemokine release. LXA4 was increased in PRP, suggesting that PRP may suppress cytokine release, limit inflammation, and, thereby, promote tissue regeneration.Platelet-Rich Plasma: Growth Factors and Pro- and Anti-Inflammatory Properties Hesham El-Sharkawy,*† Alpdogan Kantarci,* Jennifer Deady,* Hatice Hasturk,* Hongsheng Liu,* Mohammad Alshahat,† and Thomas E. Van Dyke* *Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, MA.

    †Department of Periodontology and Oral Medicine, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.

    Correspondence: Dr. Thomas E. Van Dyke, Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, 100 E. Newton St. G-107, Boston, MA 02118. Fax: 617/638-4799; e-mail: tvandyke@bu.edu.

    Background: Platelet-rich plasma (PRP) promotes regeneration of bone, presumably through the action of concentrated growth factors. However, it is not clear how PRP affects the inflammatory response. The purpose of this study was to analyze the growth factors in PRP and to study the effects of PRP on monocyte cytokine release and lipoxin A4 (LXA4) generation.

    Methods: PRP was prepared from healthy donors. Platelet-derived growth factor (PDGF)-AB, PDGF-BB, transforming growth factor-β1, insulin-like growth factor-I, fibroblast growth factor-basic (FGF-b), epidermal growth factor (EGF), vascular endothelial growth factor, interleukin-12 (p40/70), and regulated on activation, normal T-cell expressed and secreted (RANTES) levels were evaluated by enzyme-linked immunosorbent assay and bead-based multiplexing. Peripheral blood monocytes were isolated and cultured with or without PRP. Cytokine, chemokine, and LXA4 levels as well as monocyte chemotactic migration were analyzed.

    Results: Growth factors were increased significantly in PRP compared to whole blood (WB) and platelet-poor plasma. Monocyte chemotactic protein-1 (MCP-1) was suppressed significantly by PRP, whereas RANTES was increased significantly in monocyte cultures. LXA4 levels were significantly higher in PRP compared to WB. PRP stimulated monocyte chemotaxis in a dose-dependent fashion, whereas RANTES, in part, was responsible for PRP-mediated monocyte migration.

    Conclusions: PRP is a rich source of growth factors and promoted significant changes in monocyte-mediated proinflammatory cytokine/chemokine release. LXA4 was increased in PRP, suggesting that PRP may suppress cytokine release, limit inflammation, and, thereby, promote tissue regeneration.