Association of Interleukin-10 Gene Polymorphisms With Severe Generalized Chronic Periodontitis

• Autores: Pinar Sümer
• Localización: Journal of periodontology, ISSN 0022-3492, Vol. 78, Nº. 3, 2007, págs. 493-497
• Idioma: inglés
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• Resumen

  • Background: Interleukin-10 (IL-10), an anti-inflammatory cytokine, plays a role in periodontal disease by inhibiting synthesis of proinflammatory cytokines and stimulating protective antibody production. Genetic polymorphisms in the IL-10 gene might be useful as a marker to diagnose susceptibility to periodontitis. Therefore, the aim of this study was to investigate the association between IL-10 gene polymorphisms and severe generalized chronic periodontitis (CP) in a Turkish population.

    Methods: Samples of venous blood and DNA were obtained from 75 patients with severe generalized CP and 73 healthy subjects. The IL-10 promoter sequences at positions −597 and −824 were amplified by polymerase chain reaction, and polymorphisms were detected by restriction enzyme cleavage. Genotype and allele frequencies were calculated, and data were analyzed using the χ2 test.

    Results: There was a statistically significant difference in frequencies of genotypes (AA/CC + CA: P = 0.00007, odds ratio = 12.37, 95% confidence intervals = 2.74 to 7.77; CC/CA + AA: P = 0.001, odds ratio = 3.05, 95% confidence intervals = 1.47 to 6.33) and alleles (P = 0.0002, odds ratio = 2.61, 95% confidence intervals = 1.52 to 4.51) at position −597 C to A between patients with severe generalized CP and healthy controls, whereas there was no significant difference in genotypes and allele frequencies at position −824 C to T between patients with CP and healthy subjects.

    Conclusion: Within the limitations of sample selection and number, the IL-10 gene polymorphism at position −597 seems to be associated with severe generalized CP.