Effects of Ultrasound on the Proliferation and Differentiation of Cementoblast Lineage Cells
- Autores: T. Inubushi, E. Tanaka, E.B. Rego, M. Kitagawa, A. Kawazoe, A. Ohta, H. Okada, J.H. Koolstra, M. Miyauchi, T. Takata, K. Tanne
- Localización: Journal of periodontology, ISSN 0022-3492, Vol. 79, Nº. 10, 2008, págs. 1984-1990
- Idioma: inglés
- Enlaces
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Resumen
Background: The purpose of this study was to investigate the effects of low-intensity pulsed ultrasound (LIPUS) stimulation on the proliferation and differentiation of cementoblast lineage cells.
Methods: An immortalized human periodontal ligament cell line (HPL) showing immature cementoblastic differentiation was used. Cultured HPL cells were subjected to LIPUS exposure (frequency = 1 MHz; pulsed 1:4; intensity = 30 mW/cm2) or sham exposure for 15 minutes per day. Expression levels of alkaline phosphatase (ALP), type I collagen (Col-I), runt-related gene 2 (Runx2), bone sialoprotein (BSP), osteocalcin (OCN), and osteopontin (OPN) mRNA were analyzed with real-time polymerase chain reaction analysis. Furthermore, ALP activity, collagen synthesis, and protein level of Runx2 were examined after 6 days of LIPUS exposure.
Results: mRNA and protein levels of ALP, Col-I, and Runx2 were significantly increased by LIPUS exposure compared to controls, whereas BSP, OCN, and OPN mRNA expression could not be detected in HPL cells, irrespective of LIPUS exposure.
Conclusion: LIPUS enhanced ALP activity, collagen synthesis, and Runx2 expression of HPL cells, which provides important insight into the promotion of early cementoblastic differentiation of immature cementoblasts.
