The Association of Interleukin-4 Haplotypes With Chronic Periodontitis in a Czech Population

• Autores: Lydie Izakovicova Holla, Antonin Fassmann, Peter Augustin, Tomas Halabala, Vladimir Znojil, Jiri Vanek
• Localización: Journal of periodontology, ISSN 0022-3492, Vol. 79, Nº. 10, 2008, págs. 1927-1933
• Idioma: inglés
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• Resumen

  • Background: Cytokine gene polymorphisms are known to influence the susceptibility and disease course of many chronic disorders. Recently, interleukin (IL)-4 gene polymorphisms were associated with aggressive periodontitis. The aim of this study was to test differences in the distribution of the IL-4 alleles, genotypes, and haplotypes between patients with chronic periodontitis (CP) and healthy controls in a Czech population.

    Methods: The association study was conducted using an age- and smoking status–matched case-control design in patients with CP (n = 194) and healthy controls (n = 158) using the polymerase chain reaction–restriction fragment length polymorphism methods for the −590C/T, −33C/T, and intron 3 variable number tandem repeat (VNTR) variants of the IL-4 gene.

    Results: No significant differences between patients and controls were found in allele and genotype frequencies of all three polymorphisms. Nevertheless, complex analysis revealed significant differences in haplotype frequencies between the groups (P = 0.005). The haplotype T(−590)/T(−33)/allele 2 VNTR (70 base pairs)2 of the IL-4 gene was significantly more frequent in patients with CP than in controls (17.0% versus 11.0%; odds ratio = 1.85; 95% confidence interval: 1.19 to 2.87).

    Conclusion: The three polymorphisms in the IL-4 gene act in a cooperative fashion and suggest that the high-production IL-4 haplotype was associated with an increased risk for CP in the Czech population.