Using Proteins in a Bioinorganic Laboratory Experiment:: Iron Loading and Removal from Transferrin

• Autores: Erin E. Battin, Ashley Lawhon, Julia L. Brumaghim, David H. Hamilton
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 86, Nº 8 (August), 2009, págs. 969-972
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • With the increasing availability of metalloproteins it is now possible to incorporate them into bioinorganic laboratory experiments. Thus, we have developed a laboratory experiment where students use UV–vis spectroscopy to determine the rate of iron removal from transferrin by a well-known, commercially-available iron chelating drug, Deferiprone. Students gain experience in both chemical and biological laboratory techniques, while assessing the efficacy of a drug that is physiologically relevant to humans.