Using the Enzymatic Growth of Nanoparticles To Create a Biosensor. An Undergraduate Quantitative Analysis Experiment

• Autores: James Bai, Kaleyhia Flowers, Salil Benegal, Milagros Calizo, Vrudhdhi Patel, Sandra Whaley Bishnoi
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 86, Nº 6 (June), 2009, págs. 712-714
• Idioma: inglés
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• Resumen

  • This second-year analytical chemistry laboratory exposes students to biosensors and the concepts of sensitivity, linear range, and dynamic range. The use of gold nanoparticles provides a method of peroxide (Part I) and glucose (Part II) sensing that can be monitored colorimetrically using either a single wavelength or scanning UV–vis spectrophotometer. Each part of the experiment can be conducted in triplicate within a three-hour laboratory period or both combined can be conducted within the three-hour period.