Resumen de Determination of Myoglobin Stability by Circular Dichroism Spectroscopy:: Classic and Modern Data Analysis
Andrew F. Mehl, Mary A. Crawford, Lei Zhang
Few laboratory procedures describe the use of circular dichroism (CD) at the undergraduate level. To increase the number of laboratory exercises using CD, a thermal denaturation study of myoglobin using CD is described to assess protein stability. Values obtained from a more classic linear data analysis approach are consistent with data analyzed with a modern software program that "fits" the data set to a model using a non-linear approach. Both methods gave quantities consistent with reported literature values.
