Resumen de Short communication. Employment of molecular markers to develop tetraploid "supermale" asparagus from andromonoecious plants of the landrace "Morado de Huétor�"
Jose Javier Regalado González, Juan Gil, Patricia Castro, Roberto Moreno, Carlos López Encina
The aim of this work was the development of new �supermale� genotypes retaining the highest genetic diversity possible of the tetraploid asparagus landrace �Morado de Huétor�. The elite andromonoecious male HT664 of the �Morado de Huétor� asparagus landrace and the andromonoecious hybrid male HC027, obtained by crossing between this landrace and a commercial cultivar of Asparagus officinalis, were selected for self-pollination to produce possible �supermales� with genes of �Morado de Huétor� (SMHT). To confirm the hybrid nature of HC027, we characterized this genotype with EST-SSR (Expressed Sequence Tag-derived Simple Sequence Repeats) markers. We also adopted the sex-linked marker Asp1-T7 for sex determination in �Morado de Huétor� and the resulting hybrids between this landrace and other commercial cultivars. Asp1-T7 marker was used for the selection and genotyping of SMHT. �Allmale� cultivars with a different genetic background can be generated by crossing females with these new �supermale� genotypes, and the agronomical traits of these new cultivars would be very different from the �all-male� cultivars currently available in the market, making them extremely interesting for asparagus breeding programs.
