Aging and Sequential Resistance Exercise Bout Effects on Housekeeping Gene Messenger RNA Expression in Human Skeletal Muscle

• Autores: Kyle L. Sunderland, Michael D. Roberts, Vincent J. Dalbo, Chad M. Kerksick
• Localización: Journal of strength and conditioning research: the research journal of the NSCA, ISSN 1064-8011, Vol. 27, Nº. 1, 2013, págs. 1-7
• Idioma: inglés
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• Resumen

  • The purpose of this study was to investigate how age and 1 week of conventional resistance exercise affects commonly used housekeeping gene (HKG) messenger RNAs (mRNAs) in skeletal muscle. Ten college-aged (18�25 years) and 10 older (60�76 years) men completed 3 lower-body resistance exercise bouts on Monday, Wednesday, and Friday, and muscle samples were obtained before bout 1 (T1), 48 hours after the first (T2) and second bouts (T3), and 24 hours after the third bout (T4). Raw Ct values indicated that [beta]-actin and cyclophilin were more highly expressed in older vs. younger males (p < 0.01) at T1. When normalizing each HKG mRNA to the other 4 HKG mRNAs, CYC increased at T3 and glyceraldehyde-3-phosphate dehydrogenase decreased at T2 (p < 0.05) in younger men. This is one of the few studies to suggest that explicit HKG mRNAs should be used depending upon age group and resistance exercise intervention.