Radical Quenching of 1,1-Diphenyl-2-picrylhydrazyl: A Spectrometric Determination of Antioxidant Behavior.

• Autores: John M. Berger, Roshniben J. Rana, Hira Javeed, Iqra Javeed, Sandi L. Schulien
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 85, Nº 3, 2008, págs. 408-410
• Idioma: inglés
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• Resumen

  • Radical chemistry is a fundamental subject of undergraduate organic chemistry. While well described in organic textbooks, laboratory manuals rarely address this topic, focusing instead on syntheses using polar reactions. Herein, we describe a colorimetric assay using the persistent radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) to measure the antioxidant activity of common antioxidants in one (3 hour) lab period. While the laboratory procedures are designed for a second-year organic laboratory environment, minor modifications to either the experiment or the supplemental material would make this suitable for more or less advanced courses. This exercise is particularly appropriate for a course geared for nutrition or food science majors.