Absorption and Scattering Coefficients: A Biophysical-Chemistry Experiment Using Reflectance Spectroscopy.

• Autores: Gabriela B. Cordon, M. Gabriela Lagorio
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 84, Nº 7, 2007, págs. 1167-1170
• Idioma: inglés
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• Resumen

  • The scattering, S, and the absorption, K, coefficients of leaves from different plant species were determined separately as a function of wavelength from reflectance and transmittance data measured for the individual leaves. The equations that link the measurable data with the optical parameters K and S, were deduced using both the Kubelka�Munk approach and the model of the "pile of plates". In parallel, the reflectance of an optically thick sample of stacked leaves were measured and the remission function was calculated. The values of the remission function obtained at different wavelengths from stacked leaves were compared with the quotients of the optical parameters, K/S, obtained from individual leaves. Both values are predicted to be equal according the Kubelka�Munk theory. The experimental agreement shows that the pile of plates model is a good description for the optical properties of leaves.