Biocinetica y lesiones histológicas del clorhidrato de clenbuterol en modelo conejo
- Autores: Benjamín Valladares Carranza, Rómulo Bañuelos Valenzuela, Silvia Peña, Valente Velázquez Ordóñez, Jose Zamora
- Localización: Ciencias Agropecuarias Handbook T-II: Congreso Interdisciplinario de Cuerpos Académicos / coord. por Miriam Estelina Pérez Ríos, María Susana Acosta Navarrete, María Elena López Ramírez, Verónica Morales Félix, 2014, ISBN 978-607-8324-30-9, págs. 61-69
- Idioma: español
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- Dialnet Métricas: 2 Citas
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Resumen
In order to evaluate the histopathological alterations according to two different clenbuterolhy drochloride (CCL) concentrations, 15 rabbits were randomly separated into 3 groups in which 0.8 and 16 ìg/Kg of CCL respectively (T1 and T2) and for T3 (placebo) 1 gr of sugar were administered in drinking water for 28 days. In days 1, 7, 14, 21 and 28, three ml of blood were with drawn from the marginal vein to collect serum. In day 28 after blood sampling they were weighed, sacrificed and the necropsy performed. Liver and heart issue samples were collected in 10% formal in for histological procedures. CCL detection was performed in blood serum using an ELISA test (RidascreenClenbuterol FAST®). There were no evident macroscopical alterations either in the carcassnor in thet issues of the treated rabbits; never theless, in T3 rabbits there was a greater deposition of fat in the carcasses. Histologically in T2 hearts, multiple hemorrages, and nuclear pleomorphism and rows of nuclei were observed. In theliver of T1 and T2, 25 up to 50% of tissue alteration ise videnced, showing mild to moderate areas of congestion, hydropic degeneration, pyknosis, karyorrhexis and focal areas of necrosis; in 2/5 rabbits from T2 areas of necrosis, neutrophil and macrophageinfiltration surrounded by fibroblasts were observed. Only in rabbits from T1 and T2 CCL levels above detection limit were found from day 7 up to day 28, with a gradual increase of 3068 to 8100 ppt.
