Desinfección y regeneración eficiente de chayote in vitro (Sechium edule Jacq.Sw.).

• Autores: Oscar Del Ángel, Gilber Vela, Araceli Rodríguez, Miguel Gómez, Hugo García
• Localización: Ciencias Agropecuarias Handbook T-II: Congreso Interdisciplinario de Cuerpos Académicos / coord. por Miriam Estelina Pérez Ríos, María Susana Acosta Navarrete, María Elena López Ramírez, Verónica Morales Félix, 2014, ISBN 978-607-8324-30-9, págs. 23-32
• Idioma: español
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• Resumen

  • A protocol for the preparation and disinfection of chayote buds and seed material with sodium hypochlorite was tested for in vitro culturing. We defined that a total active chlorine concentration of 1.2% provided complete sterilization of the explants in a few minutes, with high percentages of survival and regenerated plants after aseptic treatment of buds (ca. 73.7%) and seeds (ca. 90%). All buds and complete seed were regenerate defficiently in MS culture media with or without plant growth regulators after the disinfection treatment.