Análisis de los microsatélites ac y ctt del gen g6pd en muestras de individuos g6pd deficientes del noroeste de México.

• Autores: Noemi García, Fred Luque, Verónica Picos, Enrique Romo, Eliakym Arámbula-Meraz
• Localización: Ciencias Naturales y Exactas Handbook T-II: Congreso Interdisciplinario de Cuerpos Académicos / coord. por Vicente Pérez García, José Luis Mínguela Ramírez, José Luis Rodríguez Muñoz, 2014, ISBN 978-607-8324-18-7, págs. 49-54
• Idioma: español
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• Resumen

  • Deficiency of Glucose-6-phosphate dehydrogenase (G6PD) is the most common enzymopathy. It is estimated to affect over 330 million people worldwide, more frequently observed in Africa, Europe, Southeast Asia and Latin America. Epidemiologic Studies in Mexico estimate a prevalence of 0.95%. The most common variants that cause deficiency include G6PD A-202A/376G, G6PD A-376G/968C and G6PD-376G/542T. We have also identified five new variants (G6PD San Luis Potosí376T, G6PD Zacatecas770T, G6PD Veracruz1094A, G6PD Yucatán1285G and G6PD Mexico DF193G). Studies in this gene have identified three highly polymorphic microsatellite repeats (CA), (AT) and (CTT) which are in linkage disequilibrium. Microsatellites are widely used as molecular markers in genetic studies because they are codominates, multiallelic and highly reproducible. The aim of this study was to identify and CTT AC microsatellites in individuals positive for G6PD deficiency originating from the north-northwest of Mexico region. Samples were included in this study correspond to a total of 27 DNA samples from individuals deficient DNA samples and 50 randomly selected individuals with negative screening. Were amplified by PCR and CTT AC G6PD gene microsatellite were separated by size using polyacrylamide gels at 10%, the results were examined using the Arlequin v3.5 software, showing variations in G6PD deficient individuals G6PD A-202A / 376G, G6PD A-376G/968C and G6PD Santamaría376G/542T relate to the most common variants of Sub-Saharan Africa. The new variant G6PD Mexico DF193A> G was not observed in any other region so you can think you have a different origin.