Examination and Manipulation of Protein Surface Charge in Solution with Electrospray Ionization Mass Spectrometry

• Autores: Deborah S. Gross, Hal Van Ryswyk
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 91, Nº 8, 2014, págs. 1240-1243
• Idioma: inglés
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• Resumen

  • Electrospray ionization mass spectrometry (ESI-MS) is a powerful tool for examining the charge of proteins in solution. The charge can be manipulated through choice of solvent and pH. Furthermore, solution-accessible, protonated lysine side chains can be specifically tagged with 18-crown-6 ether to form noncovalent adducts. Chemical derivatization of a protein in this fashion followed by �soft� ionization can provide a wealth of specific information regarding side chain identity, location, and surface chemistry. A variety of proteins are examined with ESI-MS under a range of conditions in this self-guided experiment, and the results are compared with those predicted from structural information available in the protein data bank.