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Simulated Analysis of Linear Reversible Enzyme Inhibition with SCILAB

  • Autores: Manuel Antuch, Yaquelin Ramos, Rubén Álvarez
  • Localización: Journal of chemical education, ISSN 0021-9584, Vol. 91, Nº 8, 2014, págs. 1203-1206
  • Idioma: inglés
  • Texto completo no disponible (Saber más ...)
  • Resumen
    • SCILAB is a lesser-known program (than MATLAB) for numeric simulations and has the advantage of being free software. A challenging software-based activity to analyze the most common linear reversible inhibition types with SCILAB is described. Students establish typical values for the concentration of enzyme, substrate, and inhibitor to simulate realistic kinetic data. To extract kinetic parameters, linear and nonlinear (hyperbolic) data fitting are used. The actual distinction between competitive and mixed-type inhibitions and between noncompetitive and mixed-type inhibitions is discussed on the basis of nonlinear data fitting. The activity was performed as a part of an advanced undergraduate course, �Chemical Kinetics�, and added to students� diverse preexisting knowledge.


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