Development of a reliable extraction method for the recovery of total genomic DNA from woodchip colonizing biofilm involved in gas biofiltration

• Autores: Léa Cabrol, Luc Malhautier, Janick Rocher, Franck Poly, Xavier Le Roux, Marc Jovic, Anne-Sophie Lepeuple, Jean-Louis Fanlo
• Localización: Biotechniques for air pollution control: proceedings of the 2nd International Congress on Biotechniques for Air Pollution Control, A Coruña, Spain, October 3-5, 2007 / Christian Kennes (dir. congr.), María C. Veiga (dir. congr.), 2007, ISBN 978-84-9749-258-4, págs. 517-528
• Idioma: inglés
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• Resumen

  • This preliminary study focused on a critical step for the characterization of microbial ecosystem involved in biofiltration. Two aspects of nucleic acid recovery were explored: (i) cell dispersion (three methods tested) and (ii) total DNA extraction (four methods tested). The objective is to select the optimal combination of desorption/extraction methods, allowing subsequent molecular investigations to be reliable. Three relevant criteria are used to assess extraction efficiency: DNA amount and purity, and subsequent amplification feasibility.