Resumen de A Stab-and-Roll Biopsy Technique to Maintain Gingival Epithelium for Desquamative Gingivitis: Hiroyasu Endo,* ,� ,�,� Shinichiro Aoki,* Hirotsugu Yamamoto,� and Takanori Ito*

Terry D. Rees, Edward P. Allen, Kayo Kuyama, Shinichiro Aoki, Hirotsugu Yamamoto

• Background: Desquamative gingivitis (DG) is a clinical manifestation common to several diseases. It is known that most cases of DG are caused by mucous membrane pemphigoid (MMP), oral lichen planus (OLP), or pemphigus vulgaris (PV). Early recognition and treatment of these diseases can improve the prognosis, but diagnostic delays are common in patients with DG because obtaining a diagnostic biopsy is technically challenging. A biopsy technique designed to maintain the gingival epithelium for patients with DG was developed. The usefulness of this technique is discussed.

  Methods: This study is based on a retrospective review of 27 DG cases. A stab-and-roll technique was used to obtain gingival tissue. This technique is designed to reduce lateral forces on the epithelium during the procedure and to thereby prevent the inadvertent removal of the epithelium from the biopsy specimen. A total of 52 biopsies comprising 27 for hematoxylin and eosin (H&E)�stained samples and 25 for direct immunofluorescence (DIF) testing were reviewed.

  Results: Fifty-one of the 52 biopsies (98.1%) maintained the epithelium. Only one biopsy (1.9%) showed that the epithelium was totally absent. Therefore, H&E and DIF features of 51 biopsies were analyzed. Definitive diagnoses of the diseases causing DG included MMP (13 cases), PV (eight cases), and OLP (six cases).

  Conclusions: A diagnostic biopsy was obtained from the gingiva of patients with DG using the stab-and-roll technique. The gingival epithelium was well maintained, and the relationship with the underlying connective tissue was diagnostic. In the future, this stab-and-roll biopsy technique may facilitate early diagnosis and treatment of diseases causing DG.

  KEYWORDS: Biopsy, gingival diseases, lichen planus, oral, pemphigoid, benign mucous membrane, pemphigus