Effects of Cell Lysis Treatments on the Yield of Coenzyme Q10 Following Agrobacterium tumefaciens Fermentation

• Autores: Ye Tian, Tao Yue, Jian Pei, Yao Yuan, Y. Martin Lo
• Localización: Food science and technology international = Ciencia y tecnología de alimentos internacional, ISSN-e 1532-1738, ISSN 1082-0132, Vol. 16, Nº 2, 2010, págs. 195-203
• Idioma: inglés
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• Resumen

  • The yield of CoQ10 , an intracellular product extracted from Agrobacterium tumefaciens cells is dependent on the effectiveness of cell lysis post fermentation. Various cell lysis approaches are investigated, including ultrasound, repetitive freezing/thawing, grinding and acid-heat treatment. The acid-heat combination using hydrochloric acid is found the most effective in releasing CoQ 10, followed by lactic, sulfuric, phosphoric and oxalic acids. The most significant processing parameters, namely the ratio of acid solution volume and bacteria weight (A/B ratio), incubation temperature and reaction time, are optimized by using the central composite design with a quadratic regression model built by response surface methodology. The highest CoQ 10 yield at 1.518 mg/g dry cell is attained using hydrochloric acid (3 mol/L) under optimal A/B ratio, temperature and time at 10.8 mL/g, 84.2 °C and 35.3 min, respectively.