Resumen de Cultural condition affecting the growth and production of ß-galactosidase by Bifidobacterium longum CCRC 15708 in a jar fermenter
C.A. Hsu, R.C. Yu, S.L. Lee, C.C. Chou
In the present study, the growth and production of ß-galactosidase by Bifidobacterium longum CCRC 15708 in a 5-L jar fermenter as influenced by cultivation temperature (27�42 °C), medium pH (4.5�7.5) and agitation speed (5�200 rpm) were evaluated.
In general, it was found that a cultivation temperature of 37 °C proved optimal for both growth and ß-galactosidase production by the test organism. Although the growth of the test organism was the highest in the culture with pH controlled at 4.5�6.5, the culture with pH controlled at 6.5 resulted in the highest production of ß-galactosidase. Further, agitation at 100 rpm or more was found to enhance both the growth and production of ß-galactosidase.
Fermentation conducted in a jar fermenter having the pH of the culture medium, the cultivation temperature, and the agitation speed controlled at 6.5, 37 °C, and 100 rpm, respectively, a maximum ß-galactosidase activity of 36.7 U/ml and a maximum transgalactosylation activity of 0.49 U/ml was achieved in 10 h of fermentation. There are ca 2.0 and 12.3 fold greater than the reported maximum ß-galactosidase and transgalactosylation activity, respectively, produced by B. longum CCRC 15708 in a flask culture system.
