Resumen de Formaldehyde kills spores of Bacillus subtilis by DNA damage and small, acid‐soluble spore proteins of the ααααααα/βββββββ‐type protect spores against this DNA damage

C.A. Loshon, P. Setlow, P.C. Setlow

• Killing of wild‐type spores of Bacillus subtilis with formaldehyde also caused significant mutagenesis; spores (termed α−β−) lacking the two major α/β‐type small, acid‐soluble spore proteins (SASP) were more sensitive to both formaldehyde killing and mutagenesis. A recA mutation sensitized both wild‐type and α−β− spores to formaldehyde treatment, which caused significant expression of a recA‐lacZ fusion when the treated spores germinated. Formaldehyde also caused protein–DNA cross‐linking in both wild‐type and α−β− spores. These results indicate that: (i) formaldehyde kills B. subtilis spores at least in part by DNA damage and (b) α/β‐type SASP protect against spore killing by formaldehyde, presumably by protecting spore DNA.