Laboratory-scale inactivation of African swine fever virus and swine vesicular disease virus in pig slurry

• Autores: S. M. Williams, C. Turner
• Localización: Journal of applied microbiology, ISSN 1364-5072, Vol. 87, Nº 1, 1999, págs. 148-157
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Two methods were evaluated for the inactivation of African swine fever (ASV) and swine vesicular disease (SVD) viruses in pig slurry: chemical treatment and heat treatment. The addition of NaOH or Ca(OH)2 at different concentration/time combinations at 4 °C and 22 °C was examined, as was virus stability at different temperature/time combinations. ASF virus (ASFV) was less resistant to both methods than SVD virus (SVDV). In slurry from one source, ASFV was inactivated at 65 °C within 1 min, whereas SVDV required at least 2 min at 65 °C. However, it was found that thermal inactivation depended on the characteristics of the slurry used. Addition of 1% (w/v) of NaOH or Ca(OH)2 caused the inactivation of ASFV within 150 s at 4 °C; 0·5% (w/v) NaOH or Ca(OH)2 required 30 min for inactivation. NaOH or Ca(OH)2 (1% (w/v)) was not effective against SVDV at 22 °C after 30 min, and 1·5% (w/v) NaOH or Ca(OH)2 caused inactivation of SVDV at both 4 °C and 22 °C. At higher chemical concentrations or temperatures, ASFV and SVDV inactivation was faster in slurry than in buffered medium.