Resumen de Double-strand breaks on F98 glioma rat cells induced by minibeam and broad-beam synchrotron radiation therapy

S. Gil Calle, Y. Prezado, Manuel Sabés Xamaní

• Purpose To assess the DNA damage induced by MBRT and BB radiations on glioma cells.

  Methods The analysis of fluorescent intensity emitted per nucleus was plotted versus DNA content 2 and 17 h after irradiations. At around cell-doubling time (17 h) after exposures, the remaining DNA radiation damage could be correlated with cellular death.

  Results A higher ?H2AX IF intensity per cell could be detected 2 and 17 h after MBRT when compared with BB. 17 h after MBRT, misrepaired damaged cells remained arrested in both G1 and G2 phases.

  Conclusions A pronounced G2 phase arrest was detected at 17 h after MBRT and BB. However, only after MBRT, a dose-dependent increasing number of damaged cells appeared arrested also in the G1 phase, and a higher amount of cells more prone to undergo apoptosis were detected. The threshold dose required to enhance the effectiveness of both synchrotron radiation techniques was 12 Gy.