Eosinophil depletion protects mice from tongue squamous cell carcinoma induced by 4-nitroquinoline-1-oxide

• Autores: Janine Mayra da Silva, Celso Martins Queiroz-Junior, Aline Carvalho Batista, Milene Alvarenga Rachid, Mauro Martins Teixeira, Tarcília Aparecida da Silva
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 29, Nº. 3, 2014, págs. 387-396
• Idioma: inglés
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  • Aims: Tumor-associated tissue eosinophilia (TATE) has been correlated with prognosis in oral squamous cell carcinoma (OSCC). This study aimed to investigate whether eosinophils depletion affects experimental oral carcinogenesis.

    Methods and Results: BALB/c (wild type - WT) and eosinophil-deficient (?db/GATA-1) mice were treated with the carcinogen 4-nitroquinoline-1-oxide (4NQO) in drinking water for 28 weeks. Tongues were collected for histopathological and immunohistochemical analysis, as well as for the evaluation of cytokines/chemokines by ELISA. The tongue SCC induced by 4NQO was associated with a rise in eosinophil numbers. WT-treated group showed a significantly increased incidence of SCC, with higher cytological atypia, in comparison with ?db/GATA-1 mice. Consistently, the proliferative index was higher in WT compared to the ?db/GATA-1/GATA-1-treated group. No significant changes in the concentration of CCL3, CCL11 and TNF-? were detected for both groups after 4NQO treatment. Conclusions: These results suggest that eosinophils might be responsible for the deleterious outcome of experimental tongue carcinogenesis, given that their ablation protects mice from OSCC.