Getting Things Backwards to Prevent Primer Dimers

• Autores: Mark A. Poritz, Kirk M. Ririe
• Localización: The Journal of molecular diagnostics, ISSN 1525-1578, Vol. 16, Nº. 2, 2014, págs. 159-162
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Until now, the general utility of reverse phosphoramidites has not been clear and their use has been quite limited. This is likely to change with the publication of an article by Satterfield in this issue of The Journal of Molecular Diagnostics Satterfield used both standard and reverse phosphoramidites to synthesize novel oligonucleotides that contained two 3' ends, and no 5' end. When used as primers in PCR, these molecules have the striking ability to suppress primer dimer amplification. This is an important technical contribution to nucleic acid amplification technology that also hints that oligonucleotides synthesized with different polarities will find more general use in molecular biology and molecular diagnostics.