Isothermal Strand-Displacement Polymerase Reaction for Visual Detection of the Southeast Asian - Type Deletion of a-Thalassemia

• Autores: Luxin Yu, Wei Wu, Puchang Lie, Yunhua Liu, Lingwen Zeng
• Localización: The Journal of molecular diagnostics, ISSN 1525-1578, Vol. 15, Nº. 6, 2013, págs. 776-782
• Idioma: inglés
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• Resumen

  • A rapid and reliable screening test for thalassemia carrier couples is the most effective strategy to decrease the risk of conceiving fetuses with severe thalassemia. We present an approach based on the isothermal strand-displacement polymerase reaction and the use of a lateral flow strip for the visual detection of an ?-thalassemia Southeast Asian�type deletion. This assay was used to evaluate 86 clinical samples (72 cases of Southeast Asian�type deletions and 14 cases of other types of thalassemia), and the results obtained were 100% consistent with those obtained using conventional gap-PCR. The approach thus provides a simple, sensitive, rapid, and cost-effective method for the diagnosis of thalassemia genotypes.