Resumen de Contaminación bacteriana en concentrados de plaquetas de caballos.
M.E. Álvarez, Juan Carmona, CE Giraldo
The aims ofthe study were to: 1) assess the risk ofbacterial contamination in equine platelet concentrates (PCs) obtained by the tube method under three technical conditions (laminar flow cabinet or in a c1ean laboratory environment both with burner and without burner) 2) identify the critical points ofthe process ofPCs preparation with risk ofbacterial contamination; and 3) identify the potential bacterial contaminants in the process. Bacteriological samples were taken from the skin (shaved or unshaved) of the venipuncture site in 15 horses, both before and after being disinfected; hands and throat of the operator; caps of the tubes where the blood was processed; environment where the equine blood samples were collected; laboratory environment; laminar flow cabinet; bacteriological stove; and PCs obtained under 3 technical conditions. Bacteria were isolated from non-aseptically prepared equine skin, hands and throat of the operator, and the place where the blood samples were taken. Bacteria were not isolated from tube caps, laboratory environment, laminar flow cabinet, or PCs. The isolated bacteria were normal biota from equine skin, human skin and throat, and environmental contaminants. Uncontaminated PCs can be obtained by the tube method in a c1ean laboratory environment.
