Real-time PCR analysis of tailored and non-specific inocula for the removal of dimethyl sulfide in a biotrickling filter

• Autores: Bram Sercu, Nico Boon, Sara Vander Beken, Willy Verstraete, Herman Van Langenhove
• Localización: Biotechniques for air pollution control: proceedings of the international congress Biotechniques for Air Pollution Control : A Coruña, Spain, October 5-7, 2005 / Christian Kennes (dir. congr.), María C. Veiga (dir. congr.), 2005, ISBN 84-9749-163-7, págs. 421-428
• Idioma: inglés
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• Resumen

  • In this study, different inocula were compared for the removal of 20 ppmv of dimethyl sulfide (DMS) in a biotrickling filter. Three biotrickling filters were set up in parallel, inoculated respectively with Thiobacillus thioparus TK-m, sludge and sludge + T. thioparus TK-m + Hyphomicrobium VS. Taking into account 5 criteria (length of start-up period, maximal elimination capacity, influence of intermittent feeding and peak loadings, resistance to low pH), the performance of the biotrickling filters was compared. Overall, the non-specific inoculum (sludge) showed the best performance, and a small benefit was observed by adding the two DMS degrading strains to the sludge. To quantitatively monitor the bacterial populations, realtime PCR was used with universal bacterial primers and with newly developed primers for the specific detection of the introduced strains. In HANDS++, rather constant Hyphomicrobium VS and total DNA concentrations were observed on the rings after start-up. In THIO, the applied operational changes had a clear effect on the total DNA amounts. The protocol for detection of T. thioparus needs to be further optimized, however.